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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Core-shell magnetic microporous covalent organic framework with functionalized Ti(IV) for selective enrichment of phosphopeptides
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Core-shell magnetic microporous covalent organic framework with functionalized Ti(IV) for selective enrichment of phosphopeptides

机译：核 - 壳磁性微孔共价有机骨架，具有官能化Ti（IV），用于磷酸肽的选择性富集

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摘要

Selective, sensitive and efficient phosphopeptide enrichment is significant in understanding phosphorylation- regulated processes and finding potential biomarkers. Magnetic materials have significant advantages for the separation and enrichment of modified peptides and proteins. In this work, we fabricated a core-shell magnetic Ti~(4+)-functionalized covalent organic framework composite (denoted as Fe_3O_4@SiO_2@TpPa-Ti~(4+)) to selectively capture phosphopeptides in biosamples. The specific porous structure makes high surface area, which provides space and more affinity sites to binding titanium ions. The loading capacity of titanium ions was as high as 14% (wt%). Calculated by the MS results of α-casein tryptic digests, the binding capability could reach 200 mg g~(-1). Fe_3O_4@SiO_2@TpPa-Ti~(4+) nanoparticles also demonstrate high sensitivity (above 0.2 fmol μL~(-1)) and selectivity (α-casein : BSA = 1 : 5000). 1083 phosphopeptides were identified within two LC-MS/MS replicates with 91.8% specificity (phosphopeptides/all identified peptides). These MS results demonstrate that with the aid of the magnet, this method based on the magnetic titanium-functionalized covalent organic framework composite is applicable to achieve rapid, selective and efficient phosphopeptide analysis.
机译：选择性，灵敏，高效磷酸浓缩在理解phosphorylation-调节的过程中，寻找潜在生物标志物显著。磁性材料具有分离和修饰的肽和蛋白质富集显著的优势。在这项工作中，我们制造的芯 - 壳磁的Ti〜（4 +） - 官能化的共价有机骨架复合生物样品中（表示为Fe_3O_4 @ SiO_2 @ TPPA-Ti系〜（4+）），以选择性地捕获磷酸化肽。具体多孔结构使得高表面积，其提供空间和更多的亲和力位点以结合钛离子。钛离子的装载容量为高达14％（重量％）。由α酪蛋白胰蛋白酶消化的MS结果计算，结合能力可达200​​毫克克〜（-1）。 Fe_3O_4 @ SiO_2 @ TPPA-Ti系〜（4+）的纳米颗粒也显示出高的灵敏度（高于0.2飞摩尔μL〜（-1））和选择性（α酪蛋白：BSA = 1：5000）。 1083个磷酸进行两个LC-MS / MS重复内识别与91.8％的特异性（磷酸/所有鉴定的肽）。这些MS结果表明，与所述磁铁的帮助下，基于磁钛官能共价有机骨架复合这种方法适用于实现快速，选择性的和高效的分析磷酸肽。

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