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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Aptamers vs. antibodies as capture probes in optical porous silicon biosensors
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Aptamers vs. antibodies as capture probes in optical porous silicon biosensors

机译：适体与抗体作为光学多孔硅生物传感器中的捕获探针

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摘要

Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regenerated for subsequent experiments.
机译：在过去的十年中，Aptamers成为一种有前途的生物感受器，用于生物传染型应用，具有优于常规抗体的显着优势。然而，在等效条件下比较Aptasensors和免疫传感器的实验研究是有限的，并且结果在每个生物受体类型的益处和局限性方面不确定。在目前的作品中，比较了用于检测用作模型靶标的蛋白质的适体和抗体生物的性能。将生物灭菌剂固定在用作光学换能器的纳米结构多孔硅（PSI）薄膜上，通过反射干涉傅里叶变换光谱法以实时和无标记的形式检测目标蛋白质。对于抗体，随机固定到PSI纳米结构上导致生物沉积性能差。相反，抗体的定向固定化表明，在结合速率，动态检测范围，检测范围，检测范围内，基于Aptamer的生物传感器表现出类似的生物沉积性能。在其可重复使用和可储存性方面，Aptasensor优于变形，而免疫传感器无法再生用于随后的实验。

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