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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Application of PCR fingerprinting techniques for identification anddiscrimination of Salmonella isolates
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Application of PCR fingerprinting techniques for identification anddiscrimination of Salmonella isolates

机译：PCR指纹图谱技术在沙门氏菌分离和鉴定中的应用

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摘要

Different PCR-based DNA fingerprinting techniques were evaluated for identification and discrimination of bacterial strains. Fifteen strains of Salmonella enterica subspecies enterica serotypes Typhi (10), Paratyphi A (1) and Typhimurium (3) collected over a period of 15 years from stool, blood, and urine samples and river Ganga were tested. All the strains were analysed by restriction analysis of the amplified 16s rDNA (ARDRA), random amplified polymorphic DNA (RAPD) and BOX-PCR methods. In ARDRA, strains belonging to the same species were identified by identical fingerprints; RAPD on the other hand, divided Salmonella into 9 different groups. Two ciprofloxacin (CIP)-resistant strains showed similar PCR fingerprints which were different from the rest of the 12 strains. In BOX-PCR, all the strains of Salmonella showed 6 different groups which showed the presence of a common band. Serotype Typhimurium could be placed in the same group by BOX-PCR, CIP-resistant strains also produced one characteristic extra band by BOX-PCR, It was observed that RAPD had higher discriminatory power than BOX-PCR and was a simple and rapid technique for use in epidemiological studies of isolates belonging to S. enterica.
机译：评价了不同的基于PCR的DNA指纹技术，以鉴定和区分细菌菌株。在15年的时间里，从粪便，血液和尿液样本及恒河中检测了15株肠炎沙门氏菌肠型血清型Typhi（10），Paratyphi A（1）和Typhimurium（3）。通过限制性内切酶16s rDNA（ARDRA），随机扩增多态性DNA（RAPD）和BOX-PCR方法对所有菌株进行分析。在ARDRA中，通过相同的指纹识别属于同一物种的菌株。另一方面，RAPD将沙门氏菌分为9个不同的组。两个耐环丙沙星（CIP）的菌株显示出相似的PCR指纹图谱，与其他12个菌株不同。在BOX-PCR中，所有沙门氏菌菌株均显示6个不同的组，这些组显示存在共同的条带。通过BOX-PCR可以将鼠伤寒血清型置于同一组，耐CIP的菌株也可以通过BOX-PCR产生一条特征性的额外条带，观察到RAPD具有比BOX-PCR更高的鉴别力，是一种简便快速的鉴定在肠炎链球菌分离株的流行病学研究中的应用。

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