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首页> 外文期刊>The Journal of Chemical Physics >Acceleration of DNA melting kinetics using alternating electric fields
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Acceleration of DNA melting kinetics using alternating electric fields

机译：使用交流电场加速DNA熔化动力学

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We verify both theoretically and by simulation that an AC electric field, with a frequency much higher than the dissociation rate, can significantly accelerate the dissociation rate of biological molecules under isothermal conditions. The cumulative effect of the AC field is shown to break a key bottleneck by reducing the entropy (and increasing the free energy of the local minimum) via the alignment of the molecular dipole with the field. For frequencies below a resonant frequency which corresponds to the inverse Debye dipole relaxation time, the dissociation rate can be accelerated by a factor that scales as omega(epsilon'(omega)-1)E-0(2), where omega is the field frequency, E-0 is the field amplitude, and epsilon'(omega) is the frequency-dependent real permittivity of the molecule. At large amplitudes, we find that the accelerated melting rate becomes universal, independent of duplex size and sequence, which is in drastic contrast to Ohmic thermal melting. We confirm our theory with isothermal all-atomic molecular dynamics simulation of short DNA duplexes with known melting rates, demonstrating several orders in enhancement with realistic fields. Published by AIP Publishing.
机译：我们理论上和通过模拟来验证AC电场，频率远高于解离率，可以显着加速等温条件下的生物分子的解离率。通过将分子偶极子与该领域的对准减小熵（并增加局部最小值的自由能量，将AC场的累积效果分解键瓶颈。对于低于谐振频率的频率，该频率对应于逆行偶极沉积时间，可以通过作为ω（epsilon'（omega）-1）e-0（2）的因素来加速解离率，其中欧米茄是该领域频率，E-0是场幅度，epsilon'（Omega）是分子的频率依赖性实际介电常数。在很大的振幅中，我们发现加速熔融率变得普遍，与双工尺寸和序列无关，这与欧姆热熔的复杂对比度。我们通过具有已知熔融率的短DNA双链体的等温全原子分子动力学模拟，证实了我们的理论，展示了几种与现实领域的增强订单。通过AIP发布发布。

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