...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Current Eye Research >Comparative studies of N-glycans and glycosaminoglycans present in SIRC (statens seruminstitut rabbit cornea) cells and corneal epithelial cells from rabbit eyes
【24h】

Comparative studies of N-glycans and glycosaminoglycans present in SIRC (statens seruminstitut rabbit cornea) cells and corneal epithelial cells from rabbit eyes

机译：兔眼SIRC（状态血清兔角膜）细胞和角膜上皮细胞中N-聚糖和糖胺聚糖的比较研究

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Purpose: We compared cultured Statens Seruminstitut rabbit cornea (SIRC) cells and corneal epithelial cells from rabbit eyes by analyzing their N-glycans and glycosaminoglycans (GAGs). This work is a fundamental study on the efficacy of using cultured cells instead of animals for drug development. Materials and methods: N-Glycans and GAGs from SIRC cell monolayers and corneal epithelial cells of rabbit eyes were analyzed by capillary electrophoresis (CE) and a combination of high-performance liquid chromatography (HPLC) and mass spectrometry. Results: High mannose-type glycans and a hybrid-type glycan were the common N-glycans in SIRC cells and corneal epithelial cells of rabbit eyes. Mono-fucosylated biantennary glycans with or without one N-acetylneuraminic acid residue were observed only in SIRC cells. Hyaluronic acid was the only measurable GAG in the corneal epithelial cells of rabbit eyes. In contrast, hyaluronic acid and chondroitin sulfates were abundantly present in SIRC cells. Conclusions: Profiles of both N-glycans and GAGs were conspicuously different between SIRC cells and corneal epithelial cells of rabbit eyes. This report will be useful for the evaluation of pharmaceutical candidates when animals or cultured cells are employed in drug development studies.
机译：目的：我们通过分析兔眼的N-聚糖和糖胺聚糖（GAG），比较了培养的史坦顿血清兔角膜（SIRC）细胞和角膜上皮细胞。这项工作是关于使用培养的细胞代替动物进行药物开发的功效的基础研究。材料与方法：采用毛细管电泳（CE）结合高效液相色谱（HPLC）和质谱法，分析了兔眼SIRC细胞单层和角膜上皮细胞中的N-聚糖和GAG。结果：高甘露糖型聚糖和杂合型聚糖是兔眼SIRC细胞和角膜上皮细胞中常见的N-聚糖。仅在SIRC细胞中观察到具有或不具有一个N-乙酰神经氨酸残基的单岩藻糖基化的双触角聚糖。透明质酸是兔眼角膜上皮细胞中唯一可测量的GAG。相反，透明质酸和硫酸软骨素大量存在于SIRC细胞中。结论：兔眼的SIRC细胞和角膜上皮细胞之间N-聚糖和GAGs的分布显着不同。当动物或培养的细胞用于药物开发研究中时，该报告对于评估候选药物非常有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号