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首页> 外文期刊>Current opinion in HIV and AIDS >Genotypic resistance testing in routine clinical care.
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Genotypic resistance testing in routine clinical care.

机译：常规临床护理中的基因型耐药性测试。

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PURPOSE OF REVIEW: Genotypic resistance testing has become part of routine clinical management of HIV-infected patients. Focussing on observational studies, this review looks at recent advances in this area. RECENT FINDINGS: Translation of the nucleotide sequence generated by the resistance test into clinically useful information remains a major challenge. A recent key development is the availability of therapy optimization tools to predict regimens that are most likely to achieve virological suppression. Standard genotypic resistance testing only examines protease and part of reverse transcriptase; as drugs are licensed to further targets, it has become necessary to expand the repertoire for testing. Traditionally, genotypic testing has not been attempted at viral loads less than 1000 copies/ml, but recent studies indicate that major mutations are often detected at much lower levels. Similarly, various methods have been developed for the detection of minority variants including allele-specific PCR, single-genome sequencing, and ultra-deep sequencing. SUMMARY: The technology and interpretation of genotypic resistance tests is in a phase of rapid development. It remains uncertain which of these developments will become part of routine clinical practice.
机译：审查目的：基因型耐药性检测已成为HIV感染患者常规临床管理的一部分。着重于观察性研究，本综述着眼于该领域的最新进展。最近的发现：将抗药性测试产生的核苷酸序列翻译成临床有用的信息仍然是一个重大挑战。最近的一项重要进展是可以使用治疗优化工具来预测最有可能实现病毒学抑制的方案。标准的基因型抗药性测试仅检查蛋白酶和部分逆转录酶。由于药物被许可用于进一步的目标，因此有必要扩大测试范围。传统上，尚未尝试在病毒载量低于1000拷贝/ ml时进行基因型检测，但最近的研究表明，通常在低得多的水平上检测到重大突变。同样，已开发出多种方法来检测少数变异，包括等位基因特异性PCR，单基因组测序和超深度测序。简介：基因型耐药性测试的技术和解释处于快速发展的阶段。尚不确定这些进展中的哪些将成为常规临床实践的一部分。

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