...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis
【24h】

In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis

机译：差分扫描荧光测定分析原位和敏感乳液和铝溶性止动病毒疾病病毒疫苗的敏感稳定性研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Adjuvants are important to enhance the antigens immunogenicity, but may also alter the structures of antigens. Currently off-line methods for adjuvants induced antigen alteration suffer from incomplete release and possible structural alteration of antigens. Here we investigated the differential scanning fluorimetry (DSF) as an in-situ and high-throughput strategy to analyze the stability of inactivated foot-and- mouth disease virus (iFMDV), known as 146S, in three representative adjuvants including aluminum hydroxide (AH), oil-in-water (O/W) emulsion, and water-in-oil (W/O) emulsion. Under optimized DSF conditions, the T-m referring to 146S dissociation can be detected in all three adjuvants. Using SYBR Green II as fluorescent dye enables detection of iFMDV as low as 5 mu g/mL. By comparing the T-m in different pH, three adjuvants showed different effects on 146S. Screening for excipients was successfully conducted using DSF. Sugars and glycerol increased the T-m of iFMDV in all three adjuvants, but to different degree. The stabilization by 20% (w/v) sucrose and glycerol was further verified by differential scanning calorimetry (DSC) and high performance size exclusion chromatography (HPSEC). DSF is proved also applicative for low-purity iFMDV and pre-adjuvanted iFMDV vaccines. In summary, the DSF can be a powerful tool in formulation study and vaccine quality control for inactivated virus vaccines. (C) 2020 Elsevier Ltd. All rights reserved.
机译：佐剂对于增强抗原免疫原性来说是重要的，但也可能改变抗原的结构。目前用于佐剂诱导抗原改变的离线方法患有不完全释放和可能的抗原结构改变。在这里，我们研究了差分扫描荧光测量法（DSF）作为原位和高通量策略，以分析包括氢氧化铝（αH）的三种代表性佐剂中的灭活脚口病病毒（IFMDV）的稳定性（IFMDV）（αh ），水 - 水（O / W）乳液和油油（W / O）乳液。在优化的DSF条件下，可以在所有三个佐剂中检测到146s解离的T-M。使用Sybr Green II作为荧光染料，可以检测IFMDV，低至5μg/ ml。通过比较不同pH的T-M，三种佐剂对146s显示出不同的影响。使用DSF成功进行辅料的筛选。糖和甘油在所有三个佐剂中增加了IFMDV的T-M，但不同程度。通过差示扫描量热法（DSC）和高性能尺寸排阻色谱（HPSEC）进一步验证稳定20％（w / v）蔗糖和甘油。证明了DSF也适用于低纯度IFMDV和预辅助IFMDV疫苗。总之，DSF可以是制剂研究和疫苗疫苗疫苗的强大工具。 （c）2020 elestvier有限公司保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号