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Comparison of eight Lactobacillus species for delivery of surface-displayed mycobacterial antigen

机译：八种乳杆菌物种用于递送表面展示的分枝杆菌抗原物种

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Lactobacillus spp. comprise a large group of Gram-positive lactic acid bacteria with varying physiological, ecological and immunomodulatory properties that are widely exploited by mankind, primarily in food production and as health-promoting probiotics. Recent years have shown increased interest in using lactobacilli for delivery of vaccines, mainly due to their ability to skew the immune system towards proinflammatory responses. We have compared the potential of eight Lactobacillus species, L. plantarum, L. brevis, L. curvatus, L. rhamnosus, L. sakei, L. gasseri, L. acidophilus and L. reuteri, as immunogenic carriers of the Ag85B-ESAT-6 antigen from Mycobacterium tuberculosis. Surface-display of the antigen was achieved in L. plantarum, L. brevis, L. gasseri and L. reuteri and these strains were further analyzed in terms of their in vitro and in vivo immunogenicity. All strains activated human dendritic cells in vitro. Immunization of mice using a homologous prime-boost regimen comprising a primary subcutaneous immunization followed by three intranasal boosters, led to slightly elevated IgG levels in serum in most strains, and, importantly, to significantly increased levels of antigen-specific mucosal IgA. Cellular immunity was assessed by studying antigen-specific T cell responses in splenocytes, which did not reveal proliferation as assessed by the expression of Ki67, but which showed clear antigen-specific IFN-gamma and IL-17 responses for some of the groups. Taken together, the present results indicate that L. plantarum and L. brevis are the most promising carriers of TB vaccines. (C) 2019 The Authors. Published by Elsevier Ltd.
机译：乳杆菌SPP。包括一大群革兰氏阳性乳酸菌，具有不同的生理，生态和免疫调节性质，这些特性被人类广泛利用，主要用于食品生产和促进益生菌。近年来表现出利用乳酸杆菌来递送疫苗的兴趣，主要是由于它们能够使免疫系统朝向促炎反应倾斜的能力。我们比较了八种乳杆菌，植物乳杆菌，短乳杆菌，L. curvatus，鼠李糖乳杆菌，属清酒，加氏乳杆菌，嗜酸乳杆菌和罗伊氏乳杆菌，为的Ag85B-ESAT的免疫原性载体的潜力来自结核分枝杆菌的抗原。抗原的表面显示在L.Purerarum，L.Brevis，L.Gasseri和L.Reuteri中实现，并在体外和体内免疫原性方面进一步分析这些菌株。所有菌株在体外活化人树突细胞。使用包含初级皮下免疫的同源素升压方案的小鼠免疫小鼠，然后进行三个鼻内助推器，在大多数菌株中导致血清中的IgG水平略微升高，并且重要的是，显着增加了抗原特异性粘膜IgA水平。通过研究脾细胞中的抗原特异性T细胞反应来评估细胞免疫，这不通过Ki67的表达评估而没有揭示增殖，而是显示出澄清的抗原特异性IFN-γ和IL-17对某些组的反应。在一起，目前的结果表明L.Purerarum和L.Brevis是Tb疫苗最有前景的载体。 （c）2019年作者。 elsevier有限公司出版

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