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首页> 外文期刊>Vaccine >Assessment of the efficacy of an attenuated live marker classical swine fever vaccine (Flc-LOM-BErns) in pregnant sows
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Assessment of the efficacy of an attenuated live marker classical swine fever vaccine (Flc-LOM-BErns) in pregnant sows

机译：评估减毒的现场标志古典猪瘟疫苗（FLC-LOM-BERNS）在怀孕母猪中的疗效

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摘要

Here, we constructed an attenuated live marker classical swine fever (CSF) vaccine (Flc-LOM-BErns) to eradicate CSF. This was done by taking infectious clone Flc-LOM, which is based on an attenuated live CSF vaccine virus (LOM strain), and removing the full-length classical swine fever virus (CSFV) E-rns sequences and the 3' end (52 base pairs) of the CSFV capsid. These regions were substituted with the full-length bovine viral diarrhoea virus (BVDV) E-rns gene sequence and the 3' end (52 base pairs) of the BVDV capsid gene. Sows were vaccinated with the Flc-LOM-BErns vaccine 3 weeks before insemination and then challenged with virulent CSFV at the early, mid- or late stages of pregnancy. We then examined transplacental transmission to the foetuses. Piglets born to sows vaccinated with Flc-LOM-BErns did not show vertical infection, regardless of challenge time. In addition, CSFV challenge did not affect the delivery date, weight or length of the foetus. Pregnant sows inoculated with the Flc-LOM-BErns vaccine were anti-CSF E-rns antibody-negative and anti-BVDV E-rns antibody-positive. Challenge of pregnant sows with virulent CSFV resulted in anti-CSF E-rns antibody positivity. These results strongly indicate that differential diagnosis can be conducted between the Flc-LOM-BErns vaccinated animal and virulent CSFV affected animal by detecting antibody against BVDV E-rns or CSF E-rns gene. Therefore, the Flc-LOM-BErns vaccine may fulfil the function of differential diagnosis which required for DIVA vaccine. (C) 2019 Elsevier Ltd. All rights reserved.
机译：在这里，我们构建了一个减毒的实时标记典型猪瘟（CSF）疫苗（FLC-LOM-BERN）以消除CSF。这是通过捕获传染性克隆FLC-LOM来完成的，这是基于衰减的活菌疫苗病毒（LOM菌株），并除去全长古典猪瘟病毒（CSFV）E-RNS序列和3'末端（52碱基对）CSFV衣壳。这些地区被BVDV衣壳基因的全长牛病毒腹泻病毒（BVDV）E-RNS基因序列和3'末端（52碱基对）取代。在授精前3周与FLC-LOM-BERNS疫苗接种母猪，然后在怀孕的早期，中期或晚期或晚期患者攻击毒性CSFV。然后，我们检查了胎儿的转移传动。不管挑战时间，仔猪用FLC-LOM-BERN接种疫苗的母猪没有显示出垂直感染。此外，CSFV挑战不会影响胎儿的交货日期，重量或长度。孕母猪接种用FLC-LOM-BERNS疫苗是抗CSF E-RNS抗体 - 阴性和抗BVDV E-RNS抗体阳性。怀孕母猪具有毒性CSFV的挑战导致抗CSF E-RNS抗体阳性。这些结果强烈表示通过检测BVDV E-RNS或CSF E-RNS基因的抗体，可以在FLC-LOM-Berns疫苗的动物和毒性CSFV受影响的动物之间进行鉴别诊断。因此，FLC-LOM-BERNS疫苗可以满足DIVA疫苗所需的差异诊断的功能。 （c）2019 Elsevier Ltd.保留所有权利。

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  • 来源
    《Vaccine》 |2019年第27期|共7页
  • 作者

    Lim Seong-in; Choe SeEun; Kim Ki-Sun; Jeoung Hye-Young; Cha Ra Mi; Park Gil-Soon; Shin Jihye; Park Gyu-Nam; Cho In-Soo; Song Jae-Young; Hyun Bang-Hun; Park Bong-Kyun; An Dong-Jun;

  • 作者单位

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

    Anim &

    Plant Quarantine Agcy Virus Dis Div Gimchen 39660 Gyeongbuk Do South Korea;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    Classical swine fever virus; Flc-LOM-BErns; Transplacental transmission; Pregnant sow; E-rns ELSIA;

    机译：古典猪瘟病毒;FLC-LOM-BERNS;移植传动;怀孕母猪;E-RNS ELSIA;

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