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首页> 外文期刊>Vaccine >FlaC supplemented with VAA, OmpK or OmpR as bivalent subunit vaccine candidates induce immune responses against Vibrio anguillarum in flounder (Paralichthys olivaceus)
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FlaC supplemented with VAA, OmpK or OmpR as bivalent subunit vaccine candidates induce immune responses against Vibrio anguillarum in flounder (Paralichthys olivaceus)

机译：FLAC补充有VAA，OMP或OMPR，作为二价亚基疫苗候选者诱导免疫反应在比目鱼（Paralichthys Olivaceus）的vibrio anguillarum

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In our previous study, ten candidate proteins have been identified with immunogenicity and protection against Vibrio anguillarum in flounder (Paralichthys olivaceus). Among them, FlaC is the important outer protein in the flagellum with immunogenicity; VAA, OmpK and OmpR are protection proteins against V. anguillarum. In this paper, FlaC supplemented with VAA, OmpK or OmpR as bivalent subunit vaccine candidates, and their immune response of flounder and protective effects were evaluated, respectively. Recombinant(r) proteins of FlaC were mixed with rVAA, rOmpK and rOmpR, respectively, rVAA+rFlaC (AF), rOmpK+rFlaC (KF) and rOmpR+rFlaC (RF); formalin-killed cells (FKC) or PBS were injected to flounder, respectively. After immunization, the percentages of CD3(+) T lymphocytes and surface membrane immunoglobulin-positive (sIg(+)) B lymphocytes in peripheral blood lymphocytes (PBLs), total antibodies (TA), specific antibodies against V. anguillarum (VA), specific antibodies against bivalent recombinant proteins (PA), the expression of immune-related genes and relative percent survivals (RPS) were measured, respectively. The results showed that three bivalent vaccines candidates and FKC could induce the proliferation of sIg(+) B lymphocytes and CD3(+) T lymphocytes in PBLs. The TA, VA and PA induced in bivalent vaccines candidates and FKC groups were significantly higher than that of the control group. CD3, IgM, CD4-1, CD4-2, CD8 alpha and CD8 beta genes were up-regulated. After challenge with V. anguillarum, RPS in AF, KF, RF and FKC groups exhibited 62.6 +/- 2.33%, 78.95 +/- 3.01%, 75.45 +/- 0.97%, and 56.71 +/- 2.15% respectively. The results revealed that three bivalent vaccines candidates and FKC could induce the immune response in flounder, and have good protection against V. anguillarum, and KF can be an efficient bivalent subunit vaccine candidate. (C) 2017 Elsevier Ltd. All rights reserved.
机译：在我们以前的研究中，已经用免疫原性和保护术中鉴定了十种候选蛋白质（Paralichthys Olivaceus）的vibrio anguillarum。其中，FLAC是鞭毛中的重要外部蛋白质，具有免疫原性; VAA，OMPK和OMPR是针对V. Anguillarum的保护蛋白。本文分别评估了补充有VAA，OMPK或OMPR作为二价亚基疫苗候选的FLAC及其对比目鱼的免疫应答和保护作用。 FLAC的重组（R）蛋白质与RVAA，ROMPK和ROMPR分别混合，RVAA + RFLAC（AF），ROMPK + RFLAC（KF）和ROMPR + RFLAC（RF）;将福尔马林杀死的细胞（FKC）或PBS分别注射到比目鱼中。在免疫后，CD3（+）T淋巴细胞和表面膜免疫球蛋白阳性（SIG（+））B淋巴细胞（SIG（+））B淋巴细胞在外周血淋巴细胞（PBL），总抗体（TA），针对V.Anguillarum（VA）的特异性抗体，针对二价重组蛋白（PA）的特异性抗体，分别测量免疫相关基因的表达和相对百分比存活率（RPS）。结果表明，三个二价疫苗候选物和FKC可诱导PBLS中SIG（+）B淋巴细胞和CD3（+）T淋巴细胞的增殖。在二价疫苗候选物和FKC组中诱导的TA，VA和PA显着高于对照组。上调CD3，IgM，CD4-1，CD4-2，CD8α和CD8β基因。在挑战V.Anguillarum，AF的RPS，KF，RF和FKC组中，分别显示出62.6 +/- 2.33％，78.95 +/- 3.01％，75.45 +/- 0.97％和56.71 +/- 2.15％。结果表明，三个二价疫苗候选者和FKC可以诱导比目鱼中的免疫应答，并对V.Anuillarum进行良好的保护，并且KF可以是一种有效的二价亚基疫苗候选者。 （c）2017 Elsevier Ltd.保留所有权利。

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