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首页> 外文期刊>Vaccine >Immunization with Pseudomonas aeruginosa outer membrane vesicles stimulates protective immunity in mice
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Immunization with Pseudomonas aeruginosa outer membrane vesicles stimulates protective immunity in mice

机译：用假单胞菌铜绿假单胞膜囊泡免疫刺激小鼠的保护性免疫

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摘要

Pseudomonas aeruginosa is an opportunistic pathogen responsible for a wide range of severe nosocomial and community acquired infections, these infections are major health problems for cystic fibrosis patients and immune-compromised individuals. The emergence of multidrug-resistant isolates high-lights the need to develop alternative strategies for treatment of P. aeruginosa infections. Outer membrane vesicles (OMVs) are spherical nanometer-sized proteolipids that are secreted from numerous of pathogenic Gram-negative bacteria, and a number of studies have confirmed the protective efficacy for use of OMVs as candidate vaccines. In this study, OMVs from P. aeruginosa (PA_OMVs) were isolated, formulated with aluminum phosphate adjuvant and used as a vaccine in a mouse model of acute lung infection. The results confirmed that active immunization with PA_OMVs was able to reduce bacterial colonization, cytokine secretion and tissue damage in the lung tissue, thus protecting mice from lethal challenge of P. aeruginosa. Cytokines assay validated that immunization with PA_OMVs was efficient to induce a mixed cellular immune response in mice. Further, high level of specific antibodies was detected in mice immunized with PA_OMVs, and results from opsonophagocytic killing assay and passive immunization suggested that humoral immune response may be critical for PA_OMVs mediated protection. These findings demonstrated that PA_OMVs may be served as a novel candidate vaccine for the prevention of P. aeruginosa infection. (C) 2018 Elsevier Ltd. All rights reserved.
机译：假单胞菌铜绿假单胞菌是一种机会主义病原体，负责广泛的严重医院和群落获得的感染，这些感染是囊性纤维化患者和免疫受损个体的主要健康问题。多药隔离的出现高灯需要开发用于治疗P.铜绿假单胞菌感染的替代策略。外膜囊泡（OMV）是球形纳米尺寸的蛋白质，其来自许多致病革兰氏阴性细菌分泌，并且许多研究证实了使用OMV作为候选疫苗的保护效果。在本研究中，分离出P.铜绿假单胞菌（PA_OMV）的OMV，用磷酸铝佐剂配制，并用作急性肺部感染的小鼠模型中的疫苗。结果证实，与PA_OMV的主动免疫能够降低肺组织中的细菌定植，细胞因子分泌和组织损伤，从而保护小鼠免受P.铜绿假单胞菌的致死挑战。细胞因子测定验证，使用PA_OMV的免疫有效，以诱导小鼠的混合细胞免疫应答。此外，在用Pa_omvs免疫的小鼠中检测到高水平的特异性抗体，并且Opsonophagocytic杀死测定和被动免疫的结果表明体液免疫应答对于Pa_omvs介导的保护至关重要。这些发现证明了PA_OMV可以作为预防P.铜绿假单胞菌感染的新型候选疫苗。 （c）2018年elestvier有限公司保留所有权利。

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  • 来源
    《Vaccine》 |2018年第8期|共8页
  • 作者

    Zhang Xiaoli; Yang Feng; Zou Jintao; Wu Weiru; Jing Haiming; Gou Qiang; Li Haibo; Gu Jiang; Zou Quanming; Zhang Jinyong;

  • 作者单位

    Third Mil Med Univ Southwest Hosp Dept Clin Hematol Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Southwest Hosp Dept Clin Hematol Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

    Third Mil Med Univ Dept Microbiol &

    Biochem Pharm Coll Pharm Natl Engn Res Ctr Immunol Prod Chongqing Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    Pseudomonas aeruginosa; Outer membrane vesicles; Vaccine; Protective efficacy;

    机译：假单胞菌铜绿假单胞菌;外膜囊泡;疫苗;保护疗效;

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