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首页> 外文期刊>Vaccine >Design and immune characterization of a novel Neisseria gonorrhoeae DNA vaccine using bacterial ghosts as vector and adjuvant
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Design and immune characterization of a novel Neisseria gonorrhoeae DNA vaccine using bacterial ghosts as vector and adjuvant

机译：使用细菌鬼魂作为载体和佐剂的新细胞淋病淋病性DNA疫苗的设计和免疫表征

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摘要

Gonorrhea, an important sexually transmitted disease, is becoming a growing public health problem around the globe. Vaccination is considered the best long-term approach for control of infection. In this study, we designed a novel Neisseria gonorrhoeae (N. gonorrhoeae) DNA vaccine delivered by bacterial ghosts and characterized its immune responses in vitro and in vivo. Our results demonstrate that bacterial ghosts greatly promoted BMDCs maturation and activation. Bacterial ghosts loaded with N. gonorrhoeae DNA vaccine were efficiently taken up by mouse macrophage RAW264.7 cells. Furthermore, oral immunization with the ghost vaccine candidate elicited greater CD4(+) and CD8(+) T cell responses and induced higher IgG responses than N. gonorrhoeae DNA vaccine alone. In addition, mice immunized with the vaccine candidate responded with a significant rise in bactericidal antibody titer. These results suggest that bacterial ghosts may function as a vaccine adjuvant by promoting BMDCs maturation, which in turn enhances the immune responses to the vaccine antigens. This study also highlights the potential of using bacterial ghosts as antigen delivery system in the development of an efficacious gonorrhea vaccine. (C) 2018 Elsevier Ltd. All rights reserved.
机译：淋病是一个重要的性传播疾病，正在成为全球范围内的越来越大的公共卫生问题。疫苗接种被认为是控制感染的最佳长期方法。在这项研究中，我们设计了一种新的Neisseria淋病（N.淋病）DNA疫苗，通过细菌鬼魂递送，并在体外和体内表征其免疫应答。我们的结果表明，细菌幽灵大大促进了BMDCS成熟和激活。由小鼠巨噬细胞Raw264.7细胞有效地占据含有N.淋病DNA疫苗的细菌鬼。此外，用幽灵疫苗候选的口服免疫引发更大的CD4（+）和CD8（+）T细胞反应，并仅诱导高于N.淋病疫苗的IgG应答。另外，用疫苗候选的小鼠免疫致作杀菌抗体滴度的显着升高。这些结果表明，细菌幽灵可以通过促进BMDCS成熟来用作疫苗佐剂，这反过来增强对疫苗抗原的免疫应答。本研究还突出了使用细菌鬼魂作为抗原递送系统在发育有效淋病疫苗的潜力。 （c）2018年elestvier有限公司保留所有权利。

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  • 来源
    《Vaccine》 |2018年第30期|共8页
  • 作者

    Jiao Hongmei; Yang Hui; Zhao Dan; Chen Jin; Zhanga Qianyun; Liang Jiankun; Yin Yinyan; Kong Guimei; Li Guocai;

  • 作者单位

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Med Inst Translat Med Jiangsu Key Lab Expt &

    Translat Noncoding RNA Res Yangzhou 225001 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    N. gonorrhoeae vaccine; Bacterial ghosts; Vector; Adjuvant;

    机译：N.淋病疫苗;细菌鬼;矢量;佐剂;
  • 入库时间 2022-08-19 19:15:47

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