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首页> 外文期刊>Vaccine >Reclassification of Flexibacter tractuosus NBRC 15981(T) as Marivirga harenae sp nov in the family Flammeovirgaceae
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Reclassification of Flexibacter tractuosus NBRC 15981(T) as Marivirga harenae sp nov in the family Flammeovirgaceae

机译：重新分类Flexibacter Tractuosus NBRC 15981（T）作为Marivirga Harenae Sp在Frameovirogaceae的Marivirga Harenae Sp

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摘要

Flexibacter tractuosa [Lewin, 1969] was reclassified as Marivirga tractuosa. Flexibacter tractuosus NBRC 15981(T) was reclassified herein by using a polyphasic taxonomic approach. Cells of the strain were strictly aerobic, Gram-stain-negative, slender rods, which were motile by gliding. The major respiratory quinone was menaquinone-7 and the predominant (> 5 %) cellular fatty acids were iso-C-15 : 0, iso-G-C-15 : 1, C-16 : 1 omega 7c and iso-C-17 : 0 3-OH. The polar lipid pattern indicated the presence of a phosphatidylethanolamine, several unidentified aminolipids, glycolipids and five unidentified polar lipids. The G+ C content of the genomic DNA was 35.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NBRC 15981(T) clustered with members of the genus Marivirga in the family Flammeovirgaceae of the phylum Bacteroidetes. Levels of DNA-DNA relatedness were less than 16% between strain NBRC 15981(T) and the two closely related species, Marivirga sericea NBRC 15983(T) and Marivirga tractuosa NBRC 15989(T). Strain NBRC 15981(T) could be differentiated from these type strains in the genus Marivirga based on the polar lipid pattern and the activity of alpha-chymotrypsin, as well as by aglucosidase and beta-glucosidase activity. On the basis of these results, NBRC 15981(T) is proposed as representing a novel species of the genus Marivirga, named Marivirga harenae sp. nov. The type strain is JK11(T) (= NBRC 15981(T) = NCIMB 1429(T)).
机译：Flexibacter Tractuosa [Lewin，1969]被重新分类为Marivirga Tractuosa。通过使用多相分类方法来重新分类Flexibacter Tractuosus NBRC 15981（T）。菌株的细胞严格是有氧的，革兰污染阴性细长棒，其通过滑动是动机。主要呼吸醌是menaquinone-7，主要（> 5％）细胞脂肪酸是ISO-C-15：0，ISO-GC-15：1，C-16：1 OMEGA 7C和ISO-C-17： 0 3-oh。极性脂质图案表明存在磷脂酰乙醇胺，几种未识别的氨基吡啶醇，糖脂和五种未识别的极性脂质。基因组DNA的G + C含量为35.8mol％。基于16S rRNA基因序列的系统发育分析表明，菌株NBRC 15981（T）与Marivirga属的菌株在Phylum Bacterovetes的杂志中聚集。 DNA-DNA相关性的水平小于菌株NBRC 15981（T）和两个密切相关的物种，Marivirga Sericea NBRC 15983（T）和Marivirga Tractuosa NBRC 15989（T）之间的含量小于16％。菌株NBRC 15981（T）可以基于极性脂质图案和α-脉冲苷的活性以及α-胰蛋白酶和β-葡糖苷酶活性的α-Chymotrypsin的活性不同。在这些结果的基础上，建议NBRC 15981（T）代表Marivirga属的新种，命名为Marivirga Harenae SP。 11月。类型应变是JK11（t）（= NBRC 15981（t）= ncimb 1429（t））。

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