Enterococcus crotali sp nov., isolated from faecal material of a timber rattlesnake

McLaughlin Richard W.; Shewmaker Patricia Lynn; Whitney Anne M.; Humrighouse Ben W.; Lauer Ana C.; Loparev Vladimir N.; Gulvik Christopher A.; Cochran P. A.; Dowd Scot E.

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Enterococcus crotali sp nov., isolated from faecal material of a timber rattlesnake

机译：肠球菌Crotali SP 11月。，从粪便材料的粪便材料中孤立

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A facultatively anaerobic, Gram-stain-positive bacterium, designated ETRF1(T), was found in faecal material of a timber rattlesnake (Crotalus horridus). Based on a comparative 16S rRNA gene sequence analysis, the isolate was assigned to the genus Enterococcus. The 16S rRNA gene sequence of strain ETRF1(T) showed > 97% similarity to that of the type strains of Enterococcus rotai, E. caccae, E. silesiacus, E haemoperoxidus, E. ureasiticus, E. moraviensis, E. plantarum, E. quebecensis, E. ureilyticus, E. termitis, E. rivorum and E. faecalis. The organism could be distinguished from these 12 phylogenetically related enterococci using conventional biochemical testing, the Rapid ID32 Strep system, comparative pheS and rpoA gene sequence analysis, and comparative whole genome sequence analysis. The estimated in silico DNA-DNA hybridization values were 70 %, and average nucleotide identity values were 96 %, when compared to these 12 species, further validating that ETRF1(T) represents a unique species within the genus Enterococcus. On the basis of these analyses, strain ETRF1(T) (= CCUG 65857(T) = LMG 28312(T)) is proposed as the type strain of a novel species, Enterococcus crotali sp. nov.

机译：在木材响尾蛇（Crotalus Horriidus）的粪便材料中发现了一种突变性厌氧，革兰氏阳性细菌，指定etrf1（t）。基于对比16S rRNA基因序列分析，将分离物分配给肠球菌属。菌株EtRF1（T）的16S rRNA基因序列显示出与肠球菌株，E.CACCAE，E. Silesiacus，E.Umoperoxidus，E. ulaviensis，E.Platarum，E.Platarum，E.Platarum，E.Vallarum 。魁北委，E. ureilyticus，E.脑炎，E. rivorum和E.粪便。可以使用常规的生物化学测试，快速ID32Strep系统，对比对象和RPOA基因序列分析和对比全基因组分析来区分生物体与这些12个系统源相关的肠球菌。硅DNA-DNA杂交值中的估计为＆ 70％，平均核苷酸同一性值为＆ 96％，与这些12种相比，进一步验证ETRF1（T）代表肠球菌属中的独特物种。在这些分析的基础上，提出了菌株EtRF1（T）（= CCug 65857（T）= LMG 28312（T）作为新型物种的类型菌株，肠球菌Crotali SP。 11月。

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来源
《Vaccine》 |2017年第31期|共6页
作者
McLaughlin Richard W.; Shewmaker Patricia Lynn; Whitney Anne M.; Humrighouse Ben W.; Lauer Ana C.; Loparev Vladimir N.; Gulvik Christopher A.; Cochran P. A.; Dowd Scot E.;
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作者单位

Gateway Tech Coll Gen Studies Kenosha WI 53144 USA;

Ctr Dis Control &

Prevent Atlanta GA 30329 USA;

Ctr Dis Control &

Prevent Atlanta GA 30329 USA;

Ctr Dis Control &

Prevent Atlanta GA 30329 USA;

Ctr Dis Control &

Prevent Atlanta GA 30329 USA;

Ctr Dis Control &

Prevent Atlanta GA 30329 USA;

Ctr Dis Control &

Prevent Atlanta GA 30329 USA;

St Marys Univ Minnesota Dept Biol Winona MN 55987 USA;

Mol Res LP MR DNA Shallowater TX USA;

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原文格式 PDF
正文语种 eng
中图分类医学免疫学;
关键词
Enterococcus crotali; timber rattlesnake; faecal material; Enterococcus; Crotalus horridus;

机译：肠球菌crotali;木材响尾蛇;粪便物质;肠球菌;克罗塔杆菌;

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1. Enterococcus crotali sp nov., isolated from faecal material of a timber rattlesnake [J] . McLaughlin Richard W., Shewmaker Patricia Lynn, Whitney Anne M., Vaccine . 2017,第31期

机译：肠球菌Crotali SP 11月。，从粪便材料的粪便材料中孤立
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3. Drancourtella massiliensis gen. nov., sp. nov. isolated from fresh healthy human faecal sample from South France [J] . G.A. Durand, J.-C. Lagier, S. Khelaifia, New Microbes and New Infections . 2016,第4期

机译：Massiliensis Drancourtella massiliensis gen。十一月，sp。十一月从法国南部的新鲜健康人类粪便样品中分离
4. Wautersiella enshiensis sp. nov. - selenite-reducing bacterium isolated from a selenium-mining area in Enshi, China [C] . Zhihao Qu, Linxi Yuan, Xuebin Yin, International Conference on Selenium in the Environment and Human Health . 2016

机译：Wautersiella enshiensis sp。 11月。 - 从中国恩施硒矿区分离的硒还原细菌
5. Characterization and Geochemistry of Novel Bacterium, Gen. Nov., Sp. Nov., Anaeropaleothermus hephaestusseraphensis Strain Siuc-3, Isolated 3.2 Kilometers Underground From the Driefontein Goldmine, South Africa [D] . Horton, Adam 2019

机译：新型细菌的表征和地球化学，Gen。Nov.，Sp。十一月，Anaeropaleothermus hephaestusseraphensis菌株Siuc-3，离地下3.2公里，位于南非Driefontein金矿
6. Characterization of Three New Enterococcal Species Enterococcus sp. nov. CDC PNS-E1 Enterococcus sp. nov. CDC PNS-E2 and Enterococcus sp. nov. CDC PNS-E3 Isolated from Human Clinical Specimens [O] . Maria da Glória S. Carvalho, Arnold G. Steigerwalt, Roger E. Morey, 2004

机译：表征三种新的肠球菌肠球菌。十一月CDC PNS-E1肠球菌十一月CDC PNS-E2和Enterococcus sp。十一月CDC PNS-E3从人类临床标本中分离
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机译：表征三种新的肠球菌，肠球菌。十一月CDC PNS-E1，肠球菌十一月CDC PNS-E2和Enterococcus sp。十一月CDC PNS-E3，从人类临床标本中分离

Enterococcus crotali sp nov., isolated from faecal material of a timber rattlesnake

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