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首页> 外文期刊>Journal of Animal Science >Effects of dietary L-methionine supplementation on the growth performance, carcass characteristics, meat quality, and muscular antioxidant capacity and myogenic gene expression in low birth weight pigs
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Effects of dietary L-methionine supplementation on the growth performance, carcass characteristics, meat quality, and muscular antioxidant capacity and myogenic gene expression in low birth weight pigs

机译:膳食L-蛋氨酸补充对低出生体重猪生长性能,胴体特性,肉质,肌源性抗氧化能力和肌源性基因表达的影响

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摘要

The aim of the present study was to investigate the effects of dietary L-Met supplementation on the growth performance, carcass characteristics, meat quality, and muscular antioxidant capacity and myogenic gene expression in low birth weight (LBW) pigs. Thirty normal birth weight (NBW) and 60 LBW female piglets were selected at birth. In each litter, after weaning, 1 of the LBW piglets (LBW-CON group) and 1 of the NBW piglets (NBW-CON group) were fed the basal diets and 1 LBW littermate was fed the basal diet supplemented with L-Met (LBW-MET group). Thus, all pigs were distributed into groups of 3 treatments x 6 replicates (pens) x 5 piglets per replicate up to 180 d of age. Compared with NBW-CON pigs, LBW-CON pigs had decreased ADG (P = 0.004) and ADFI (P 0.001) during the postweaning period and greater backfat thickness (P = 0.015) at slaughter. In addition, LBW-CON pigs exhibited compromised meat quality, as evidenced by a greater drip loss at 24 h postmortem(P = 0.037) and a lower pH at 45 min postmortem ntents of malondialdehyde (MDA; P = 0.046) and protein carbonyl (P = 0.028) in the LM. The LBW pigs fed the L-Metsupplemented diets had a greater amount of reduced glutathione (GSH; P = 0.009) but a lower level of MDA (P = 0.015) in the LM compared with the LBW-CON pigs. l-Methionine supplementation increased the pH at 24 h postmortem (pH(24h)) value (P = 0.004) but reduced the drip loss at both 24 (P = 0.016) and 48 h (P = 0.005) postmortem of LBW-MET pigs in comparison with the LBW-CON pigs. The l-Met-supplemented diets increased the S-adenosyl-l-methionine content (P = 0.006), DNA methyltransferase activity (P = 0.007), and CpG methylation levels of the sites + 27 (P = 0.008) and + 160 (P = 0.009) of myostatin (MSTN) exon 1 but decreased the mRNA expression of MSTN (P = 0.011) in the LM of the LBW-MET group compared with the LM of the LBW-CON group. Additionally, when compared with the LBW-CON group, the area of LM (P = 0.037) was significantly increased in the LBW-MET group, in parallel with the upregulated mRNA abundance of myogenin (P = 0.025), myocyte enhancer factor 2A (P = 0.036), and myocyte enhancer factor 2D (P = 0.015). In conclusion, l-Met supplementation increases pH(24h) and decreases drip loss in the LM of LBW-MET pigs, along with a greater GSH content but a lower MDA accumulation. Also, the LBW-MET pigs showed a greater LM area, which may be associated with the improved expression of myogenic genes.
机译:本研究的目的是探讨膳食L-MET补充对低出生体重(LBW)猪生长性能,胴体特征,肉质,肌源性抗氧化能力和肌源性基因表达的影响。在出生时选择三十个正常的分娩重量(NBW)和60乳头仔猪。在每个垃圾中,在断奶后,将1BW仔猪(LBW-CON组)和1个NBW仔猪(NBW-CON组)中的1次加入基础饮食,1 LBW凋落物喂入补充L-MET的基础饮食( LBW-Met组)。因此,将所有猪分发给3种处理x 6重复(笔)×5仔猪,每次复制至180天。与NBW-CON猪相比,在切换期间,LBW-CON猪在切换时期的ADG(P = 0.004)和ADFI(P <0.001)下降,并且在屠宰时更大的背脂厚度(P = 0.015)。此外,LBW-CON猪表现出损害的肉质,如24小时的滴水损失所证明的(P = 0.037)和较低的pH值在45分钟的丙二醛(MDA; P = 0.046)和蛋白质羰基( p = 0.028)在LM中。喂养L-Metsupplemented饮食的LBW猪具有更大的谷胱甘肽(GSH; P = 0.009),但与LM与LM与LM-CON猪相比,LM的较低水平的MDA(P = 0.015)。 L-甲硫氨酸补充剂在24小时后的pH值增加(pH(24小时))值(p = 0.004),但在24(p = 0.016)和48h(p = 0.005)后的LBW-Met猪的滴滴涕(P = 0.005)减少与LBW-CON猪相比。 L-MET补充的饮食增加了S-腺苷-1-甲硫氨酸含量(p = 0.006),DNA甲基转移酶活性(P = 0.007),以及位点的CPG甲基化水平+ 27(p = 0.008)和+ 160(与LM-CON组的LM相比,P = 0.009)肌抑素(MSTN)外显子1,但在LM-Met组的LM中降低MSTN(P = 0.011)的mRNA表达。另外,与LBW-CON组相比,LM(P = 0.037)的面积在LBW-Met组中显着增加,与肌菌素的上调mRNA丰富(p = 0.025),肌细胞增强剂因子2a平行( P = 0.036),肌细胞增强子因子2D(P = 0.015)。总之,L-MET补充增加了pH(24h)并降低了LMS型猪的LM中的滴落损失,以及更大的GSH含量,但MDA含量较低。此外,LBW-Met猪显示出更大的LM区域,其可能与肌原基因的改善表达相关。

著录项

  • 来源
    《Journal of Animal Science》 |2017年第9期|共12页
  • 作者单位

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物学;
  • 关键词

    antioxidant capacity; L-methionine; low birth weight; meat quality; myogenic gene expression; pig;

    机译:抗氧化能力;L-蛋氨酸;出生体重低;肉质;肌源性基因表达;猪;

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