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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Comparative Transcriptome Analysis Revealing the Effect of Light on Anthocyanin Biosynthesis in Purple Grains of Wheat
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Comparative Transcriptome Analysis Revealing the Effect of Light on Anthocyanin Biosynthesis in Purple Grains of Wheat

机译：比较转录组分析揭示了光对小麦紫色粮食中的花青素生物合成效果

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To study the mechanism of anthocyanin-biosynthesis regulation, we examined light-regulated gene expression involved in anthocyanin biosynthesis in purple grains of wheat. Ten kinds of anthocyanins were identified from a purple-grained wheat cultivar by HPLC-ESI-MS/MS analysis. Libraries constructed from the total RNA of purple grains under light (L) or dark (D) conditions for 15 or 20 days were sequenced. In total, 1874 differentially expressed genes (DEGs) were identified in L20 vs L15, 1432 DEGs were identified in D20 vs D15, 862 DEGs were identified in D 15 vs L15, and 1786 DEGs were identified in D20 vs L20. DEG functional enrichments suggested that light-signal transduction is critical to anthocyanin biosynthesis. The 911 DEGs referred to as light-regulated DEGs (LDEGs) involved a number of genes in anthocyanin biosynthesis, transcription regulation, sugar- and calcium-signaling pathways, and hormone metabolism. These findings laid the foundation for future studies on the regulatory mechanisms of anthocyanin biosynthesis in purple grains of wheat.
机译：为研究花青素 - 生物合成调控的机制，我们检查了紫色粮食中涉及花青素生物合成中的明显基因表达。通过HPLC-ESI-MS / MS分析从紫色颗粒品种中鉴定了10种花青素。测序由紫色（L）或暗（D）条件下的紫色晶粒的总RNA构建的文库进行测序15或20天。总共在L20VS L15中鉴定了1874个差异表达基因（DEGS），在D20 VS D15中鉴定了1432次，在D 15 Vs L15中鉴定出862次，并在D 20 Vs L20中鉴定1786次。 DEG功能性富集表明光信号转导对花青素生物合成至关重要。被称为测量的DEG（LDEG）的911℃涉及花青素生物合成，转录调控，糖和钙信号通路和激素代谢中的许多基因。这些调查结果为未来的紫色谷物中的花青素生物合成调节机制研究奠定了基础。

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