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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Prevention of Necrotizing Enterocolitis through Milk Polar Lipids Reducing Intestinal Epithelial Apoptosis
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Prevention of Necrotizing Enterocolitis through Milk Polar Lipids Reducing Intestinal Epithelial Apoptosis

机译：通过牛奶极性脂质降低肠上皮细胞凋亡预防坏死性小肠结肠炎

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摘要

Neonatal necrotizing enterocolitis (NEC) is a common and devastating disease. The objective of this research was to investigate the protective mechanisms of milk polar lipids (MPLs) on the attenuation of lipopolysaccharides (LPS)-induced intestinal inflammation and apoptosis. MPLs were extracted from buttermilk and analyzed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A neonatal NEC rat model was used to investigate the effects of MPLs on NEC and its underlying mechanisms. Hematoxylin-eosin (H&E) staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay were used to observe intestinal morphological changes and intestinal epithelial cell apoptosis, which showed that MPLs could reduce NEC symptoms and intestinal apoptosis. The expressions of IL-6, IL-8, and TNF-alpha in the MPL group was significantly downregulated (P < 0.05), and the expression levels of IL-10 were significantly upregulated (P < 0.05). At the same time, MPLs also significantly reduced (P < 0.05) activation of the LPS-induced TLR4/NF-kappa B signaling pathway. Furthermore, MPLs inhibit apoptosis by reducing the expressions of Bax, caspase-9, and caspase-3 and by increasing the expression of Bcl-2. In conclusion, MPLs could reduce NEC symptoms in mice by inhibiting cell inflammation and protecting against intestinal apoptosis.
机译：新生儿坏死性小肠结肠炎（NEC）是一种常见而毁灭性的疾病。本研究的目的是研究牛奶极性脂质（MPLS）对脂多糖（LPS）诱导的肠炎和细胞凋亡的衰减的保护机制。从酪乳中提取MPLS并使用超级性液相色谱 - 串联质谱（UPLC-MS / MS）分析。新生儿NEC大鼠模型用于研究MPLS对NEC及其潜在机制的影响。苏木精 - 曙红（H＆E）染色和末端脱氧核苷酸转移酶介导的DUTP-BIOTIN缩乳末端标记（TUNEL）测定法观察肠形态变化和肠上皮细胞凋亡，表明MPLS可以降低NEC症状和肠道凋亡。 MPL组中IL-6，IL-8和TNF-α的表达显着下调（P <0.05），显着上调IL-10的表达水平（P <0.05）。同时，MPLS也显着降低（P <0.05）LPS诱导的TLR4 / NF-KAPPA发信号通路的激活。此外，MPLS通过减少Bax，Caspase-9和Caspase-3的表达并通过增加Bcl-2的表达来抑制细胞凋亡。总之，MPLS通过抑制细胞炎症和免受肠内凋亡，可以减少小鼠中的NEC症状。

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  • 作者

    Yang Yujie; Zhang Tao; Zhou Guangyu; Jiang Xiaoxiao; Tao Mingxuan; Zhang Jiaxin; Zeng Xiaoqun; Wu Zhen; Pan Daodong; Guo Yuxing;

  • 作者单位

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

    Ningbo Univ Coll Food &

    Pharmaceut Sci Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Coll Food &

    Pharmaceut Sci Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Coll Food &

    Pharmaceut Sci Ningbo 315211 Zhejiang Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Dept Food Sci &

    Technol Nanjing 210023 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    neonatal necrotizing enterocolitis; milk polar lipids; cytokines; NF-kappa B pathway; apoptosis;

    机译：新生儿坏死性小肠结肠炎;牛奶极性脂质;细胞因子;NF-κB途径;细胞凋亡;

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