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首页> 外文期刊>Journal of Applied Polymer Science >Immobilization of carbonic anhydrase on polyethylenimine/dopamine codeposited membranes
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Immobilization of carbonic anhydrase on polyethylenimine/dopamine codeposited membranes

机译：聚乙胺/多巴胺类多巴胺类多巴胺膜对碳酸酐酶的固定化

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摘要

Carbonic anhydrase (CA) catalyzing CO2 hydration has an important application in carbon capture, and its immobilization is very significant. Here, CA was covalently linked by glutaraldehyde (GA) to the surface of poly(vinylidene fluoride) (PVDF) and polyethylene (PE) membranes, which were previously modified via a simple codeposition of polyethyleneimine (PEI) and dopamine (DA). The effects of the modification conditions were investigated, and the membranes were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. The immobilization process was optimized, and the catalytic properties of immobilized CA were studied. The results show that the optimal mass ratio of PEI and DA was 1:1 and the deposition time was 10-12 h, at which the surface amino group density could reach 1.278 x 10(-7) and 1.397 x 10(-7) mol/cm(2) for PVDF and PE, respectively. For enzyme immobilization, the optimal CA and GA concentrations were 0.2 mg/mL and 0.1 wt %, and a maximum activity recovery of about 53% and 76% could be achieved for PVDF-attached CA and PE-attached CA, respectively. Their K-m values were 10.62 mM and 8.6 mM, and the corresponding K-cat/K-m values were 132.2 M-1 s(-1) and 312.9 M-1 s(-1). After immobilization, the storage stability and reusability of CA were much improved. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47784.
机译：碳酸酐酶（CA）催化CO 2水合在碳捕集的一个重要应用，其固定是非常显著。这里，CA是通过共价戊二醛（GA）链接到聚的表面（偏二氟乙烯）（PVDF）和聚乙烯（PE）膜，以前经由聚乙烯亚胺的共沉积简单（PEI）和多巴胺（DA）改性。的变形条件的影响进行了调查，和将所述膜的特征在于傅里叶变换红外光谱和扫描电子显微镜。的固定化过程进行了优化，并固定CA的催化性能进行了研究。结果表明，PEI和DA的最佳质量比为1：1和沉积时间为10-12小时，在此表面的氨基密度可能达到1.278×10（-7）和1.397×10（-7）用于分别PVDF和PE，摩尔/厘米（2）。用于酶固定化，最优CA和GA浓度为0.2毫克/毫升和0.1重量％，和大约可以为PVDF-附CA来实现53％和76％和PE-附CA，最大活动恢复。其K-m个值分别为10.62 mM和8.6毫米，和相应的K-猫/ K-m的值分别为132.2 M-1 S（-1）和312.9 M-1 S（-1）。固定后，CA的储存稳定性和可重用性都大大提高。 （c）2019 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2019年，136，47784。

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  • 来源
    《Journal of Applied Polymer Science》 |2019年第30期|共9页
  • 作者

    Sun Jing; Wang Caihong; Wang Yanzi; Ji Shuxin; Liu Wenfang;

  • 作者单位

    Beijing Inst Technol Dept Chem Engn Sch Chem &

    Chem Engn Liangxiang Higher Educ Pk Beijing 102488 Peoples R China;

    Beijing Inst Technol Dept Chem Engn Sch Chem &

    Chem Engn Liangxiang Higher Educ Pk Beijing 102488 Peoples R China;

    Beijing Inst Technol Dept Chem Engn Sch Chem &

    Chem Engn Liangxiang Higher Educ Pk Beijing 102488 Peoples R China;

    Beijing Inst Technol Dept Chem Engn Sch Chem &

    Chem Engn Liangxiang Higher Educ Pk Beijing 102488 Peoples R China;

    Beijing Inst Technol Dept Chem Engn Sch Chem &

    Chem Engn Liangxiang Higher Educ Pk Beijing 102488 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 高分子化合物工业（高聚物工业）;
  • 关键词

    bioengineering; catalysts; coatings; membranes;

    机译：生物工程;催化剂;涂料;膜;

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