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首页> 外文期刊>Journal of Applied Polymer Science >Improving collagen extraction through an alternative strategy based on succinic anhydride pretreatment to retain collagen's triple-helix structure
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Improving collagen extraction through an alternative strategy based on succinic anhydride pretreatment to retain collagen's triple-helix structure

机译：基于琥珀酸酐预处理的替代策略改善胶原蛋白提取，以保留胶原蛋白的三螺旋结构

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The improvement of the extraction yield of collagen while retaining its triple-helix structure continues to represent a significant challenge for the high-value utilization of collagen. In this study, pigskin was pretreated by succinic anhydride via the chemical linking of additional carboxylic groups to epsilon amino groups with a conversion degree of 90.2% to obtain pretreatment acid-pepsin-solubilized collagen (PAPC). The pretreatment by succinic anhydride increased the tropocollagen distance from 1.39 to 1.42nm. This permitted acid and pepsin to more easily enter into the fiber clearance and, thus, improved the collagen extraction yield by 9.6%. Furthermore, X-ray diffraction, Fourier transform infrared spectroscopy, circular dichroism, ultrasensitive differential scanning calorimetry, and atomic force microscopy analysis demonstrated that the triple-helix conformation of PAPC was well-maintained. The equilibrium surface tension and isoelectric point of PAPC were 57.48 mN/m and 4.01, respectively; this suggested that the PAPC had surface activity and better solubility in a neutral pH solution. The novelty of PAPC lay in its facilitating fibroblast proliferation, and no extra cytotoxicity was introduced into the collagen after pretreatment. According to these results, our study revealed that succinic anhydride pretreatment as an alternative strategy retained the triple-helix structure of collagen and improved its extraction ratio; this might be a feasible, yet promising paradigm for the high-value utilization of collagen. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45424.
机译：胶原蛋白提取产率的提高仍在继续为胶原蛋白的高价值利用产生重大挑战。在该研究中，通过琥珀酸酐通过附加羧基的化学链接预处理猪皮以换氨基的化学连接，转化度为90.2％以获得预处理酸胃蛋白溶解的胶原（PAPC）。通过琥珀酸酐的预处理从1.39到1.42nm增加了对流凝块距离。这种允许的酸和胃蛋白酶更容易进入纤维间隙，从而将胶原萃取产率提高了9.6％。此外，X射线衍射，傅里叶变换红外光谱，圆形二色性，超敏差分扫描量热法和原子力显微镜分析表明PAPC的三螺旋构象保持着良好。 PAPC的平衡表面张力和等电点分别为57.48mn / m和4.01;这表明PAPC在中性pH溶液中具有表面活性和更好的溶解性。 PAPC新颖性介绍其促进成纤维细胞增殖，并且在预处理后没有将额外的细胞毒性引入胶原蛋原中。根据这些结果，我们的研究表明，琥珀酸酸酐预处理作为替代策略保留了胶原的三螺旋结构，提高了其提取率;这可能是胶原蛋白的高价值利用率的可行性，但很有希望的范式。 （c）2017 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2017,134,45424。

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