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首页> 外文期刊>Journal of Alloys and Compounds: An Interdisciplinary Journal of Materials Science and Solid-state Chemistry and Physics >Synthesis, characterization and evaluation of Co3O4 nanoparticles toxicological effect; synthesized by cochineal dye via environment friendly approach
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Synthesis, characterization and evaluation of Co3O4 nanoparticles toxicological effect; synthesized by cochineal dye via environment friendly approach

机译：Co3O4纳米颗粒毒理学效应的合成，表征和评价; 通过Cochineal Dye通过环境友好的方法合成

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摘要

Magnetic oxide nanoparticles (MNPs) have been extensively utilized in a wide range of biomedical applications, in order to improve the efficiency of the MNPs in therapeutic applications, there for in the present work high quality cobalt oxide magnetic nanoparticles were synthesized with a green solid state approach using cochineal powder (a natural and environmentally friendly source) as a biosynthetic precursor. This method is novel, green, simple, one step, surfactant free, solvent free, low temperature and fast. A natural complex of Co(NO3)(2) - cochineal prepared and thermally decomposed in 400, 500 and 600 degrees C. The synthesized-nanoparticles were characterized by; scanning electron microscope (SEM), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), energy dispersive spectrometry (EDS) and vibrating sample magnetometer (VSM). MTT assay performed to determine the cytotoxicity and anticancer effect of the Co3O4 nanoparticles against 4T1 cell lines. Acridine orange/ethidium bromide staining methods, DAPI staining was applied in order to investigate the mechanism of cell death. The effect of the Co3O4 nanoparticles on oxidative stress studied as well using the H2DCF-DA assay used for the reactive oxygen species (ROS) generation study. The results demonstrate that the Co3O4 nanoparticles significantly reduced the survival percentage of 4T1 cells of breast cancer with that IC50 of 2 mg/ml. The dominant mechanism of cell death was apoptosis, along with an increase in cellular oxidative stress. Therefore, the Co3O4 nanoparticles prepared here can be used as a chemotherapeutic agent against invasive breast cancer cells. However, further in vivo study is required to confirm its effect. (C) 2019 Elsevier B.V. All rights reserved.
机译：磁氧化物纳米颗粒（MNP）已被广泛用于各种生物医学应用中，为了提高MNP在治疗应用中的效率，在本工作中，用绿色固态合成高质量的氧化钴磁性纳米颗粒使用科基粉（天然和环保来源）作为生物合成前体的方法。该方法是新颖的，绿色，简单，一步，无表面活性剂，无溶剂，低温，快速。 CO（2）（2） - 制备的天然络合物（2） - 制备和在400,500和600℃下热分解。合成纳米颗粒的特征在于;扫描电子显微镜（SEM），X射线衍射图（XRD），透射电子显微镜（TEM），能量分散光谱（EDS）和振动样品磁力计（VSM）。 MTT测定进行以确定CO 3 O4纳米颗粒对4T1细胞系的细胞毒性和抗癌作用。吖啶橙/乙锭染色方法，应用DAPI染色以研究细胞死亡的机制。 Co3O4纳米颗粒对使用用于反应性氧（ROS）生成研究的H2DCF-DA测定研究研究的氧化应力。结果表明，CO3O4纳米颗粒具有2mg / ml的IC50的4T1细胞的生存率显着降低了4T1细胞的存活百分比。细胞死亡的显性机制是凋亡，随着细胞氧化应激的增加。因此，这里制备的CO 3 O 4纳米颗粒可用作侵入乳腺癌细胞的化学治疗剂。然而，进一步在体内研究需要确认其效果。 （c）2019 Elsevier B.v.保留所有权利。

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