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首页> 外文期刊>Journal of Cell Science >The golgin Pplmh1 mediates reversible cisternal stacking in the Golgi of the budding yeast Pichia pastoris
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The golgin Pplmh1 mediates reversible cisternal stacking in the Golgi of the budding yeast Pichia pastoris

机译：Golgin PPLMH1在萌芽酵母Pichia Pastoris的Golgi中介导可逆的棺材堆叠

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摘要

The adhesive force for cisternal stacking of Golgi needs to be reversible - to be initiated and undone in a continuous cycle to keep up with the cistemal maturation. Microscopic evidence in support of such a reversible nature of stacking, in the form of TGN peeling,' has been reported in various species, suggesting a potential evolutionarily conserved mechanism. However, knowledge of such mechanism has remained sketchy. Here, we have explored this issue in the budding yeast Pichia pastoris which harbors stacked Golgi. We observed that deletion of GRIP domain golgin P. pastoris (Pp)lMH1 increases the peeling of late cistema, causing unstacking of the Golgi stack. Our results suggest that the Pplmh1 dimer mediates reversible stacking through a continuous association-dissociation cycle of its GRIP domain to the middle and late Golgi cistema under the GTP hydrolysis-based regulation of Arl3-Arl1 GTPase cascade switch. The reversible cisternal stacking function of Pplmh1 is independent of its vesicle-capturing function. Since GRIP domain proteins are conserved in plants, animals and fungi, it is plausible that this reversible mechanism of Golgi stacking is evolutionarily conserved.
机译：用于颅骨堆叠的GOLGI的粘合力需要可逆 - 在连续循环中启动和撤消以跟上静脉成熟。以各种物种报道，以支持TGN剥离形式的堆叠这种可逆性的微观证据，建议潜在的进化保守机制。然而，对这种机制的了解仍然粗略。在这里，我们在崭露头角的酵母Pichia Pastoris中探讨了这个问题，其中留下了Golgi。我们观察到，夹具域名高尔金P. Pastoris（PP）LMH1的缺失增加了晚脊柱岩的剥离，从而导致Golgi堆叠的脱钩。我们的研究结果表明，PPLMH1二聚体通过其抓地域的连续关联 - 解离循环介导可逆堆叠在ARL3-AR11 GTPAse级联开关的GTP水解调节下，通过其抓地域的连续结合循环循环到中间和晚期的肠蠕动。 PPLMH1的可逆剖面堆叠功能与其凹陷捕获功能无关。由于抓握域蛋白在植物，动物和真菌中保守，因此卓越的Golgi堆叠的可逆机制是流化的保守。

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