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首页> 外文期刊>Journal of Cell Science >Catabolic activity of osteoblast lineage cells contributes to osteoclastic bone resorption in vitro
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Catabolic activity of osteoblast lineage cells contributes to osteoclastic bone resorption in vitro

机译：成骨细胞谱系细胞的分解剂活性有助于体外骨质体骨吸收

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摘要

Osteoblast lineage cells in human bone were recently shown to colonize eroded bone surfaces and to closely interact with osteoclasts. They proved to be identical to reversal cells and are believed to differentiate into bone-forming osteoblasts thereby coupling resorption and formation. However, they also exert catabolic activity that contributes to osteoclastic bone resorption, but this has not received much attention. Herein, we used co-cultures of primary human osteoblast lineage cells and human osteodasts derived from peripheral blood monocytes to investigate whether a catabolic activity of osteoblast lineage cells could impact on osteoclastic bone resorption. Through a combination of immunofluorescence, in situ hybridization and time-lapse experiments, we show that MMP-13-expressing osteoblast lineage cells are attracted to and closely interact with bone-resorting osteoclasts. This close interaction results in a strong and significant increase in the bone resorptive activity of osteoclasts - especially those making trenches. Importantly, we show that osteoclastic bone resorption becomes sensitive to inhibition of matrix metalloproteinases in the presence, but not in the absence, of osteoblast lineage cells. We propose that this may be due to the direct action of osteoblast-lineage-derived MMP-13 on bone resorption.
机译：最近显示人骨中的成骨细胞谱系细胞，以殖民化侵蚀的骨表面，并与疏口细胞密切相关。他们证明与逆转细胞相同，并且被认为分化为骨形成的成骨细胞，从而耦合吸收和形成。然而，他们还发挥了促进骨质体骨吸收的分解代谢活动，但这并没有受到很多关注。在此，我们使用了源自外周血单核细胞的原发性人成骨细胞谱系细胞和人骨骨代摩特的共培养物，以研究成骨细胞谱系细胞的分解活性是否可能会影响骨细胞骨吸收。通过免疫荧光的组合，原位杂交和延时实验，我们表明MMP-13表达的成骨细胞谱系细胞被吸引并与骨骼促进骨质体密切相关。这种密切的相互作用导致骨骨细胞的骨吸收活性的强烈和显着增加 - 尤其是那些制作沟渠的骨膜体积。重要的是，我们表明，骨细胞骨骨吸收对骨毛细胞谱系细胞的存在而不是在不存在的情况下对基质金属蛋白酶的抑制变敏。我们提出这可能是由于骨盆谱系衍生的MMP-13对骨吸收的直接作用。

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