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首页> 外文期刊>Journal of Cell Science >Myosin V regulates synaptopodin clustering and localization in the dendrites of hippocampal neurons
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Myosin V regulates synaptopodin clustering and localization in the dendrites of hippocampal neurons

机译：肌球蛋白V在海马神经元树枝状内调节突触泛黄素聚类和定位

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摘要

The spine apparatus (SA) is an endoplasmic reticulum-related organelle that is present in a subset of dendritic spines in cortical and pyramidal neurons, and plays an important role in Ca2+ homeostasis and dendritic spine plasticity. The protein synaptopodin is essential for the formation of the SA and is widely used as a maker for this organelle. However, it is still unclear which factors contribute to its localization at selected synapses, and how it triggers local SA formation. In this study, we characterized development, localization and mobility of synaptopodin clusters in hippocampal primary neurons, as well as the molecular dynamics within these clusters. Interestingly, synaptopodin at the shaft-associated clusters is less dynamic than at spinous clusters. We identify the actin-based motor proteins myosin V (herein referring to both the myosin Va and Vb forms) and VI as novel interaction partners of synaptopodin, and demonstrate that myosin V is important for the formation and/or maintenance of the SA. We found no evidence of active microtubule-based transport of synaptopodin. Instead, new clusters emerge inside spines, which we interpret as the SA being assembled on-site.
机译：脊柱装置（SA）是一种内质网相关细胞器，其存在于皮质和金字塔神经元的树突刺的子集中，并在Ca2 +稳态和树突脊柱可塑性中起重要作用。蛋白质Synaptopodin对于形成SA并广泛用作该细胞器的制造商是必不可少的。但是，目前尚不清楚哪些因素有助于其在所选突触处的本地化，以及如何触发本地SA形成。在这项研究中，我们在海马原代神经元中表征了Synaptopodin簇的发展，定位和迁移率，以及这些簇内的分子动力学。有趣的是，轴相关簇的Synaptopodin在棘突中的动态较小。我们鉴定基于肌动蛋白的电动机蛋白肌蛋白v（本文指的是肌球蛋白VA和VB形式）和VI作为Synaptopodin的新型相互作用伙伴，并证明肌蛋白V对SA的形成和/或维持是重要的。我们发现没有证据表明突触肽基于活性微管的转运。相反，新集群出现在内部刺，我们将其解释为在现场组装的SA。

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