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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Correlating the phase settling behavior of aqueous-organic solvent systems in a centrifugal partition chromatograph
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Correlating the phase settling behavior of aqueous-organic solvent systems in a centrifugal partition chromatograph

机译：在离心分配色谱仪中的水性有机溶剂体系相沉降行为的相关性

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摘要

The prediction of the performance of a Centrifugal Partition Chromatography (CPC) is a difficult but desirable task. The partitioning of the sample, as well as the fluid dynamical phenomena dispersion, coalescence, and stationary phase retention have to be individually understood. Therefore, the phase settling behavior of different aqueous-organic solvent systems and with this, the dependency of the stationary phase retention in CPC was investigated in this study. On the one hand, batch settling experiments were performed, and the settling velocity of aqueous-organic solvent systems was investigated. With this it was possible to correlate the stationary phase retention in CPC in both operating modes. For descending mode operation a high settling velocity of the lower phase and for ascending mode a high settling velocity of the upper phase is needed for a stable operation and a high stationary phase retention.
机译：对离心分配色谱（CPC）的性能预测是难以但理想的任务。 样品的分区，以及流体动力学现象分散，聚结和固定相保留必须单独理解。 因此，在本研究中研究了不同水 - 有机溶剂系统的相位沉降行为，并用这一点，在CPC中研究了固定相保留的依赖性。 一方面，进行批量沉降实验，研究了水性 - 有机溶剂体系的沉降速度。 因此，可以将CPC中的固定相位保留在两个操作模式中相关。 对于下降模式操作，下阶段的高沉降速度和用于上升模式的高稳定速度是稳定的操作和高静止相位保留。

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