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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Modeling counter-current chromatography with non-ideal injection
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Modeling counter-current chromatography with non-ideal injection

机译：用非理想注射模拟逆流色谱法

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In general counter-current chromatography systems, there are several off-column fittings between injector and column inlet, such as bends, valves, connecting tubes and joints. Due to these off-column fittings, the sample will diffuse in the mobile phase and form an irregular distribution when it flows from the injector to the column inlet. Thus, the concentration distribution of the solutes at the column inlet is a continuous curve (called the injection profile). As some previous research reveals, it is necessary to input actual injection profile into the simulation model to mimic elution profile. Therefore, we built a non-ideal CCC model whose initial value is from the actual injection profile, and validated the rationality of this model with iteration method. The simulation analysis of different injection profiles shows the conditions whereby a discrete injection profile can replace the actual injection profile in the non-ideal CCC model for accurate simulation elution. Simulation elution under such conditions reveal that non-ideal injection model can reflect the relationship between the injection profile and elution profile, and help to explain the reasons of irregular change in elution profile, like the tailed peak and flat peak. (C) 2020 Elsevier B.V. All rights reserved.
机译：在一般的逆流色谱系统中，喷射器和柱入口之间有几个偏心件，例如弯曲，阀门，连接管和接头。由于这些离子柱配件，样品将在流动相中漫射并在从喷射器流到柱入口时形成不规则的分布。因此，柱入口处的溶质的浓度分布是连续曲线（称为注射曲线）。正如以前的一些研究所揭示的那样，必须将实际的注射轮廓输入到模拟模型中以模拟洗脱轮廓。因此，我们构建了一个非理想的CCC模型，其初始值来自实际注射配置文件，并通过迭代方法验证了该模型的合理性。不同喷射轮廓的仿真分析显示了离散喷射轮廓可以代替非理想CCC模型中的实际注射轮廓以准确模拟洗脱的条件。在这种条件下模拟洗脱表明，非理想注射模型可以反映喷射曲线和洗脱谱之间的关系，并有助于解释洗脱轮廓的不规则变化的原因，如尾峰和扁平峰值。 （c）2020 Elsevier B.v.保留所有权利。

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