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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >One injection to profile the chemical composition and dual-antioxidation activities of Rosa chinensis Jacq.
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One injection to profile the chemical composition and dual-antioxidation activities of Rosa chinensis Jacq.

机译：一个注射率先介绍罗莎中华人民园的化学成分和双抗氧化活动。

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摘要

An on-line high-performance liquid chromatography-diode-array-detector-electrospray ionization ion-trap time-of-flight mass spectrometry total antioxidant capacity detection (HPLC-DAD-ESI-IT-TOF-MS-TACD) system was applied for the identification and evaluation of antioxidants in Rosa chinensis Jacq., an edible flower in food industry and a widely used traditional Chinese medicine. With the help of this platform, the HPLC fingerprint, mass fragmentations, and sample activity profiles against 1,1-diphenylpicryl-2-hydrazyl radical (DPPH center dot) and ferric reducing antioxidant power (FRAP) were recorded after one injection. Using this technique, 80 compounds were separated and identified by their LC/MS behaviors with the assistance of standard compounds. In addition, 11 different Rosa chinensis Jacq. samples were profiled and then quantified for their DPPH center dot and FRAP activities. Interestingly, a total of 52 compounds showed antioxidative effects against DPPH center dot and 61 were active against FRAP. The results demonstrated that the on-line system is a powerful technique for antioxidant discovery in Rosa chinensis Jacq. and other food resources. (C) 2019 Elsevier B.V. All rights reserved.
机译：在线高效液相色谱 - 二极管阵列 - 检测器 - 电喷雾电离离子捕获飞行时间质谱总光谱总体抗氧化能力检测（HPLC-DAD-ESI-IT-TOF-MS-TACD）系统罗莎中华民神经糖抗氧化剂的鉴定及评价。，食品工业中可食用的花卉和广泛使用的中医。在该平台的帮助下，一次注射后，记录了HPLC指纹，质量碎片，质量碎片和样品活性曲线对1,1-二苯基吡啶基-2-肼基 - 肼（DPPH中心点）和还原抗氧化能力（FRAP）。使用该技术，通过标准化合物的辅助分离并通过其LC / MS行为分离和鉴定80种化合物。此外，11种不同的Rosa Chinensis Jacq。分析样品，然后为其DPPH中心点和FRAP活动量化。有趣的是，总共52种化合物对DPPH中心点的抗氧化作用显示出抗氧化作用，61例对抗FRAP。结果表明，在线系统是罗莎中华民族南罗萨·苏格兰省抗氧化毒性的强大技术。和其他食物资源。 （c）2019 Elsevier B.v.保留所有权利。

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