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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Operating mode selection for the separation of intermediately-eluting components with countercurrent and centrifugal partition chromatography
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Operating mode selection for the separation of intermediately-eluting components with countercurrent and centrifugal partition chromatography

机译：用逆流和离心分配色谱分离中间洗脱组分的操作模式选择

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摘要

Two operating modes that can be used for the separation of intermediately-eluting target components from complex mixtures using solid support-free liquid-liquid chromatography (LLC) include batch injections and trapping multiple dual mode (trapping MDM). Batch injections offer the advantage of simpler equipment and process design, while the trapping MDM process, although more complex, can provide improved resolution at low separation factors. In this study, a thorough comparison of batch injection and trapping MDM performance with respect to productivity is made using simulations for a wide range of separation tasks (target component/impurity separation factors; minimum purity and yield requirements). For each separation task, the maximum productivities obtained by the two operating modes were separately determined with respect to their independent parameters. The results indicate that trapping MDM can be used to extend the application of LLC down to separation factors as low as 1.1, with its use being most advantageous for separation factors of approximately 1.3. However, the simpler batch injection process remains preferable for separation factors around or greater than 1.5. (C) 2019 Elsevier B.V. All rights reserved.
机译：可以使用使用固体载体液 - 液相色谱（LLC）从复杂混合物中分离中间洗脱靶分成分的两种操作模式包括批量喷射并捕获多个双模式（捕获MDM）。批量喷射提供更简单的设备和工艺设计的优势，而捕获MDM过程虽然更复杂，但可以在低分离因子下提供改进的分辨率。在该研究中，使用模拟用于各种分离任务（目标组件/杂质分离因子;最小纯度和产量要求，彻底比较分批喷射和捕获MDM性能。对于每个分离任务，通过两种操作模式获得的最大生产率是关于其独立参数的单独确定的。结果表明，捕获MDM可用于将LLC的施加延伸到低至1.1的分离因子，其使用是大约1.3的分离因子的最有利因子。然而，对于周围或大于1.5的分离因子，更简单的批量喷射过程​​保持优选。 （c）2019 Elsevier B.v.保留所有权利。

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