NMR studies of protein folding and binding in cells and cell-like environments

Smith Austin E.; Zhang Zeting; Pielak Gary J.; Li Conggang

首页> 外文期刊>Current Opinion in Structural Biology >NMR studies of protein folding and binding in cells and cell-like environments

【24h】

NMR studies of protein folding and binding in cells and cell-like environments

机译：核磁共振研究蛋白质在细胞和类细胞环境中的折叠和结合

获取原文

获取原文并翻译 | 示例

获取外文期刊封面封底 >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Proteins function in cells where the concentration of macromolecules can exceed 300 g/L. The ways in which this crowded environment affects the physical properties of proteins remain poorly understood. We summarize recent NMR-based studies of protein folding and binding conducted in cells and in vitro under crowded conditions. Many of the observations can be understood in terms of interactions between proteins and the rest of the intracellular environment (i.e. quinary interactions). Nevertheless, NMR studies of folding and binding in cells and cell-like environments remain in their infancy. The frontier involves investigations of larger proteins and further efforts in higher eukaryotic cells.

机译：蛋白质在大分子浓度可能超过300 g / L的细胞中起作用。这种拥挤的环境影响蛋白质物理特性的方式仍然知之甚少。我们总结了最近在细胞和体外在拥挤条件下进行的蛋白质折叠和结合的基于NMR的研究。可以从蛋白质与其余细胞内环境之间的相互作用（即三元相互作用）来理解许多观察结果。然而，在细胞和类细胞环境中进行折叠和结合的NMR研究仍处于初期阶段。前沿领域涉及对更大蛋白质的研究以及对更高等真核细胞的进一步努力。

著录项

来源
《Current Opinion in Structural Biology》 |2015年第null期|共10页
作者
Smith Austin E.; Zhang Zeting; Pielak Gary J.; Li Conggang;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词

相似文献

外文文献
中文文献
专利

1. NMR studies of protein folding and binding in cells and cell-like environments [J] . Smith Austin E., Zhang Zeting, Pielak Gary J., Current Opinion in Structural Biology . 2015,第Null期

机译：核磁共振研究蛋白质在细胞和类细胞环境中的折叠和结合
2. Intestinal Fatty Acid Binding Protein: The Folding Mechanism As Determined by NMR Studies [J] . Michael E. Hodsdon, Carl Frieden Biochemistry . 2001,第3期

机译：肠道脂肪酸结合蛋白：折叠机制由核磁共振研究确定。
3. Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and ~(19)F NMR [J] . Hua Li, Carl Frieden Proceedings of the National Academy of Sciences of the United States of America . 2007,第29期

机译：使用慢折叠突变体和〜（19）F NMR观察肠道脂肪酸结合蛋白折叠的顺序步骤
4. What makes IgG binding domain of protein L fold up to native state: a simulation study with physical oriented energy functions coupled to topology induced terms [C] . Seung Yup Lee, Fujitsuka, Y., . 2003

机译：是什么使蛋白L的IgG结合结构域折叠成天然状态：具有物理定向的能量函数与拓扑诱导的术语耦合的模拟研究
5. 19F NMR of SH3 Folding and Peptide Binding in Cells and Cell-Like Environments [D] . Stadmiller, Samantha S. 2020

机译：19F的SH3折叠和肽在细胞和细胞样环境中结合的肽结合
6. Dynamic NMR spectral analysis and protein folding: identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR. [O] . I J Ropson, C Frieden 1992

机译：动态NMR光谱分析和蛋白质折叠：通过19F NMR鉴定大鼠肠道脂肪酸结合蛋白的高密度折叠中间体。
7. Dynamic NMR spectral analysis and protein folding: identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR. [O] . Ropson, I J, Frieden, C 1992

机译：动态NMR光谱分析和蛋白质折叠：通过19F NMR鉴定大鼠肠道脂肪酸结合蛋白的高密度折叠中间体。
8. Structural and Functional Studies of Fungal Cellulose-Binding Domains by NMR211 Spectroscopy [R] . Mattinen, M. L. 1998

机译：通过NmR211光谱学对真菌纤维素结合域的结构和功能研究

NMR studies of protein folding and binding in cells and cell-like environments

摘要

著录项

相似文献

相关主题

期刊订阅