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首页> 外文期刊>Journal of Molecular Biology >Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria
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Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria

机译：导致蛋白质淀粉膜过生的途径的光学导航

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摘要

Modulation of liquid-liquid and liquid-hydrogel phase transitions is central to avoid the cytotoxic aggregation of proteins in eukaryotic cells, but knowledge on its relevance in bacteria is limited. Here the power of optogenetics to engineer proteins as light-responsive switches has been used to control the balance between solubility and aggregation for LOV2-WH1, a chimera between the plant blue light-responsive domain LOV2 and the bacterial prion-like protein RepA-WH1. These proteins were first linked by fusing, as a continuous a-helix, the C-terminal photo-transducer Ja helix in LOV2 with the N-terminal domain-closure a1 helix in RepA-WH1, and then improved for light-responsiveness by including mutations in the Ja moiety. In the darkness and in a crowded solution in vitro, L0V2-WH1 nucleates the irreversible assembly of amyloid fibers into a hydrogel. However, under blue light illumination, LOV2-WH1 assembles as soluble oligomers.
机译：液体和液态水凝胶相转变的调节是避免真核细胞中蛋白质细胞毒性聚集的中央，但是关于其在细菌中的相关知识有限。 这里，光源对工程蛋白作为光响应开关的功率已经用于控制LOV2-WH1的溶解度和聚集之间的平衡，植物蓝光响应域LOV2和细菌朊病毒蛋白质REPA-WH1之间的嵌合性 。 这些蛋白质首先通过熔合，作为连续A-Helix，在Lov2中的C末端光换能器Ja螺旋，通过Repa-Wh1中的N末端域闭合A1螺旋，然后通过包括透明响应性改善 JA部分中的突变。 在黑暗和体外溶液中，L0V2-WH1将淀粉样纤维的不可逆组装成水凝胶。 然而，在蓝光照明下，LOV2-WH1组装为可溶性低聚物。

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