Force-Profile Analysis of the Cotranslational Folding of HemK and Filamin Domains: Comparison of Biochemical and Biophysical Folding Assays

Kemp Grant; Kudva Renuka; de la Rosa Andrs; von Heijne Gunnar

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Force-Profile Analysis of the Cotranslational Folding of HemK and Filamin Domains: Comparison of Biochemical and Biophysical Folding Assays

机译：麻疹和丝瘤域分类折叠的力分析：生物化学和生物物理折叠测定的比较

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We have characterized the cotranslational folding of two small protein domains of different folds-the alpha-helical N-terminal domain of HemK and the beta-rich FLN5 filamin domain-by measuring the force that the folding protein exerts on the nascent chain when located in different parts of the ribosome exit tunnel (force-profile analysis, or FPA), allowing us to compare FPA to three other techniques currently used to study cotranslational folding: real-time FRET, photo induced electron transfer, and NMR. We find that FPA identifies the same cotranslational folding transitions as do the other methods, and that these techniques therefore reflect the same basic process of cotranslational folding in similar ways. (C) 2019 Elsevier Ltd. All rights reserved.

机译：我们已经表征了两个不同折叠的两种小蛋白质结构域的分致折叠 - α-螺旋N-末端结构域的α-致脂肪的FLN5菲素域 - 通过测量折叠蛋白在位于新生链上施加的力核糖体出口隧道的不同部分（力型分析或FPA），允许我们将FPA比较目前用于研究分氯化物折叠的其他技术：实时褶皱，照片诱导电子转移和NMR。我们发现FPA识别与其他方法相同的配体折叠转换，因此这些技术以类似的方式反映了与双转抗性折叠的相同基本过程。（c）2019 Elsevier Ltd.保留所有权利。

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来源
《Journal of Molecular Biology》 |2019年第6期|共7页
作者
Kemp Grant; Kudva Renuka; de la Rosa Andrs; von Heijne Gunnar;
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作者单位

Stockholm Univ Dept Biochem &

Biophys SE-10691 Stockholm Sweden;

Stockholm Univ Dept Biochem &

Biophys SE-10691 Stockholm Sweden;

Stockholm Univ Dept Biochem &

Biophys SE-10691 Stockholm Sweden;

Stockholm Univ Dept Biochem &

Biophys SE-10691 Stockholm Sweden;

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原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
cotranslational folding; HemK; FLN5; arrest peptide;

机译：CoTranslational折叠;麻疹;FLN5;捕获肽;

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1. Force-Profile Analysis of the Cotranslational Folding of HemK and Filamin Domains: Comparison of Biochemical and Biophysical Folding Assays [J] . Grant Kemp, Renuka Kudva, Andres de la Rosa Gunnar von Heijne Journal of Molecular Biology . 2019,第16期

机译：麻疹和丝瘤域分类折叠的力分析：生物化学和生物物理折叠测定的比较
2. Cotranslational folding studies of spectrin and Ig-like domains show folding occurs close to the ribosome [J] . Steward Annette, Nilsson Ola B., Nickson Adrian A., Protein Science: A Publication of the Protein Society . 2016,第Suppla1期

机译：血影蛋白和Ig样结构域的共翻译折叠研究表明折叠发生在核糖体附近
3. Co-Translational Folding Trajectory of the HemK Helical Domain [J] . Mercier Evan, Rodnina Marina V Biochemistry . 2018,第25期

机译：HEMK螺旋域的共转化折叠轨迹
4. Cotranslational Protein Folding with L-systems [C] . Gemma B. Danks, Susan Stepney, Leo S. D. Caves European Conference of Artificial Life . 2015

机译：用L-Systems折叠配转酯蛋白质
5. I. Biophysical studies on PIN WW domains: A model for understanding folding and stability of beta-sheet proteins. II. Towards understanding templated beta-sheet self-assembly. [D] . Deechongkit, Songpon. 2003

机译：I. PIN WW域的生物物理研究：了解β-折叠蛋白折叠和稳定性的模型。二。旨在了解模板化的Beta-sheet自组装。
6. Biophysical Analysis of the MHR Motif in Folding and Domain Swapping of the HIV Capsid Protein C-Terminal Domain [O] . Rebeca Bocanegra, Miguel Ángel Fuertes, Alicia Rodríguez-Huete, 2015

机译：MHR母题在HIV衣壳蛋白C末端域的折叠和域交换中的生物物理分析
7. Force-Profile Analysis of the Cotranslational Folding of HemK and Filamin Domains: Comparison of Biochemical and Biophysical Folding Assays [O] . Grant Kemp, Renuka Kudva, Andrés de la Rosa, 2019

机译：麻疹和丝瘤域分类折叠的力分析：生物化学和生物物理折叠测定的比较

Force-Profile Analysis of the Cotranslational Folding of HemK and Filamin Domains: Comparison of Biochemical and Biophysical Folding Assays

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