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首页> 外文期刊>Journal of Molecular Biology >Structure of the Centromere Binding Factor 3 Complex from Kluyveromyces lactis
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Structure of the Centromere Binding Factor 3 Complex from Kluyveromyces lactis

机译：来自Kluyveromyces Lactis的Centromere结合因子3复合物的结构

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摘要

Kinetochores are the multiprotein complexes that link chromosomal centromeres to mitotic-spindle microtubules. Budding yeast centromeres comprise three sequential "centromere-determining elements", CDEI, II, and III. CDEI (8 bp) and CDEIII (similar to 25 bp) are conserved between Kluyveromyces lactis and Saccharomyces cerevisiae, but CDEII in the former is twice as long (160 bp) as CDEII in the latter (80 bp). The CBF3 complex recognizes CDEIII and is required for assembly of a centromeric nucleosome, which in turn recruits other kinetochore components. To understand differences in centromeric nucleosome assembly between K. lactis and S. cerevisiae, we determined the structure of a K. lactis CBF3 complex by electron cryomicroscopy at similar to 4 angstrom resolution and compared it with published structures of S. cerevisiae CBF3. We show differences in the pose of Ndc10 and discuss potential models of the K. lactis centromeric nucleosome that account for the extended CDEII length. (C) 2019 Published by Elsevier Ltd.
机译：KINETOCHORES是将染色体焦点链接到有丝分裂纺锤体微管的多曲线复合物。萌芽酵母焦粒子包含三个顺序的“焦点 - 测定元素”，CDEI，II和III。在Kluyveromyces乳房和酿酒酵母中，Cdei（8bp）和Cdeiii（类似于25 bp），但前者的Cdeii是后者（80 bp）中的Cdeii的两倍（160bp）。 CBF3复合物识别CdeIII，并且需要组装焦细核小体，这反过来促进其他动力学组分。为了了解K.乳酸和酿酒酵母之间的焦化核心组件的差异，我们通过电子冷冻镜检查的K.乳酸CBF3复合物的结构与4埃分辨率相比，并将其与S.Cerevisiae CBF3的公开结构进行比较。我们在NDC10的姿势中显示出差异，并讨论K.乳酸乳糜置核细胞的潜在模型，其占延长的CDEII长度。 （c）2019年由elestvier有限公司发布

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