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首页> 外文期刊>Journal of Molecular Biology >Oleic Acid Induces MiR-7 Processing through Remodeling of Pri-MiR-7/Protein Complex
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Oleic Acid Induces MiR-7 Processing through Remodeling of Pri-MiR-7/Protein Complex

机译：油酸通过重塑Pri-miR-7 /蛋白质复合物进行诱导miR-7加工

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摘要

MicroRNAs (miRs) play a vital role in governing cell function, with their levels tightly controlled at transcriptional and post-transcriptional levels. Different sets of RNA-binding proteins interact with primary miRs (pri-miRs) and precursor-miR transcripts (pre-miRs), controlling their biogenesis post-transcriptionally. The Hu antigen R (HuR)-mediated binding of Musashi homolog2 (MSI2) to the conserved terminal loop of pri-miR-7 regulates the levels of brain-enriched miR-7 formation in a tissue-specific manner. Here, we show that oleic acid (OA) inhibits the binding of proteins containing RNA recognition motifs (RRM) to the conserved terminal loop of pri-miR-7. Using electrophoretic mobility shift assays in HeLa cell extracts, we show that OA treatment disrupts pre-miR/protein complexes. Furthermore, OA rescues in vitro processing of pri-miR-7, which is otherwise blocked by HuR and MSI2 proteins. On the contrary, pri-miR-16 shows reduced processing in the presence of OA. This indicates that OA may inhibit the binding of other RRM-containing protein/s necessary for miR-16 processing. Finally, we demonstrate that OA induces mature miR-7 production in HeLa cells. Together, our results demonstrate that OA can regulate the processing of pri-miRs by remodeling their protein complexes. This provides a new tool to study RNA processing and a potential lead for small molecules that target the miR-7 biogenesis pathway. (C) 2017 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
机译：MicroRNA（MIRS）在治疗细胞功能中发挥重要作用，其水平在转录和转录后水平中紧密控制。不同的RNA结合蛋白与原发性mir（pri-mir）和前体 - mir转录物（前mir）相互作用，在转录后控制它们的生物生成。 Hu抗原R（HUR）介导的usashi homologoolog2（msi2）与Pri-miR-7的保守末端环的结合调节了组织特异性方式的脑富集的miR-7形成水平。在此，我们表明油酸（OA）抑制含有RNA识别基序（RRM）的蛋白质的结合Pri-miR-7的保守终端环。在HeLa细胞提取物中使用电泳迁移率移位测定，我们表明OA治疗破坏了MIR /蛋白质复合物。此外，OA拯救了Pri-miR-7的体外加工，其被围绕HUR和MSI2蛋白封闭。相反，PRI-MIR-16显示在OA存在下的降低处理。这表明OA可以抑制MIR-16加工所需其他含RRM含蛋白质的结合。最后，我们证明OA在HeLa细胞中诱导成熟miR-7产生。我们的结果表明，OA可以通过重塑蛋白质复合物来调节Pri-MiR的加工。这提供了一种研究RNA处理的新工具和针对靶向miR-7生物发生途径的小分子的潜在铅。 （c）2017年作者。由elsevier有限公司发布这是CC下的开放式访问文章（http://creativecommomons.org/licenses/by/4.0/）。

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