Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells

Li Miao; Dong Qiang; Zhu Bing

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Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells

机译：Aurora激酶B在哺乳动物细胞的有丝分裂期间在丝网中磷酸化组蛋白H3.3

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Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro. (C) 2017 Elsevier Ltd. All rights reserved.

机译：组蛋白H3.3的丝氨酸31在哺乳动物细胞的有丝分裂期间磷酸化，已被证明影响染色体隔离。我们建立了一种筛选系统，用于通过使用靶向720个人激酶的siRNA文库来鉴定候选激酶H3.3S31，并通过独立激酶抑制剂筛网证实了结果。 Aurora激酶B是两个屏幕中击中的最佳候选人。此外，在体外用核体底物生物化学证实H3.3S31上的极光B的激酶活性。（c）2017 Elsevier Ltd.保留所有权利。

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《Journal of Molecular Biology》 |2017年第13期|共4页
作者
Li Miao; Dong Qiang; Zhu Bing;
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Peking Univ Sch Life Sci Peking Univ Univ Nat Inst Biol Sci Joint Grad Pro Beijing 100081;

Chinese Acad Sci CAS Ctr Excellence Biomacromol Inst Biophys Natl Lab Biomacromol Beijing;

Chinese Acad Sci CAS Ctr Excellence Biomacromol Inst Biophys Natl Lab Biomacromol Beijing;

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正文语种 eng
中图分类分子生物学;
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1. Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells [J] . Li Miao, Dong Qiang, Zhu Bing Journal of Molecular Biology . 2017,第13期

机译：Aurora激酶B在哺乳动物细胞的有丝分裂期间在丝网中磷酸化组蛋白H3.3
2. CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells [J] . F. Lyn Chan, Fiona?T.?M. Chang, James D. R.?McGhie, Nucleic acids research . 2015,第5期

机译：CHK1驱动的组蛋白H3.3丝氨酸31磷酸化对于人类ALT癌细胞的染色质维持和细胞存活很重要
3. Expression of Aurora-B kinase and phosphorylated histone H3 in hepatocellular carcinoma. [J] . Sistayanarain A, Tsuneyama K, Zheng H, Anticancer Research: International Journal of Cancer Research and Treatment . 2006,第5Appa期

机译：Aurora-B激酶和磷酸化组蛋白H3在肝细胞癌中的表达。
4. Towards Understanding the Roles of Histone H3 serine 10 Phosphorylation in Mouse Embryonic Stem Cells [C] . Shu Lin, Benjamin A. Garcia American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：了解组蛋白H3丝氨酸10磷酸化在小鼠胚胎干细胞中的作用
5. Determining the Regulation and Requirements of the Three Aurora Kinases in Mammalian Female Meiosis [D] . Nguyen, Alexandra Legeza. 2018

机译：确定哺乳动物女性减数分裂三种极光激酶的调控和要求
6. CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells [O] . Fiona T. M. Chang, F. Lyn Chan, James D. R. McGhie, 2015

机译：CHK1驱动的组蛋白H3.3丝氨酸31磷酸化对于人类ALT癌细胞的染色质维持和细胞存活很重要
7. Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation of cdc25C by p34cdc2 on sites phosphorylated at mitosis. [O] . U. Strausfeld, A. Fernandez, J.P. Capony, 1994

机译：用人CDC25C微调P34CDC2蛋白激酶在哺乳动物细胞中的激活。 P34CDC2在有丝分裂中磷酸化位点的P34CDC2对CDC25C的现有磷酸化的要求。
8. Defects in Histone H3.3 Phosphorylation and ATRX Recruitment to Misaligned Chromosomes during Mitosis Contribute to the Development of Pediatric Glioblastomas. [R] . Hinchcliffe, E. H. 2015

机译：组蛋白缺陷H3.3磷酸化和aTRX招募到有丝分裂期间错位的染色体有助于小儿胶质母细胞瘤的发展。

Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells

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