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首页> 外文期刊>Journal of Molecular Biology >The NS1 Protein from Influenza Virus Stimulates Translation Initiation by Enhancing Ribosome Recruitment to mRNAs
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The NS1 Protein from Influenza Virus Stimulates Translation Initiation by Enhancing Ribosome Recruitment to mRNAs

机译：来自流感病毒的NS1蛋白通过增强MRNA的核糖体募集来刺激翻译引发

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Abstract The non-structural protein NS1 of influenza A viruses exerts pleiotropic functions during infection. Among these functions, NS1 was shown to be involved in the control of both viral and cellular translation; however, the mechanism by which this occurs remains to be determined. Thus, we have revisited the role of NS1 in translation by using a combination of influenza infection, mRNA reporter transfection, and in vitro functional and biochemical assays. Our data show that the NS1 protein is able to enhance the translation of virtually all tested mRNAs with the exception of constructs bearing the Dicistroviruses Internal ribosome entry segment (IRESes) (DCV and CrPV), suggesting a role at the level of translation initiation. The domain of NS1 required for translation stimulation was mapped to the RNA binding amino-terminal motif of the protein with residues R38 and K41 being critical for activity. Although we show that NS1 can bind directly to mRNAs, it does not correlate with its ability to stimulate translation. This activity rather relies on the property of NS1 to associate with ribosomes and to recruit them to target mRNAs. Graphical Abstract Display Omitted Highlights ? NS1 enhances translation at the level of initiation. ? NS1 binds to mRNAs and ribosomes. ? Only the binding of NS1 to ribosomes is required for translation stimulation.
机译：摘要流感病毒的非结构蛋白NS1在感染期间施加抗性功能。在这些功能中，显示NS​​1参与病毒和细胞翻译的控制;然而，发生这种情况的机制仍有待确定。因此，我们通过使用流感感染，mRNA报告转染和体外功能和生物化学测定的组合重新判断了NS1在翻译中的作用。我们的数据表明，NS1蛋白能够通过构建Dicistroviruses内部核糖体入口区段（IRESES）（DCV和CRPV）的构建体除以构建的构建体，提高几乎所有测试的mRNA的翻译，表明在翻译启动水平上的作用。翻译刺激所需的NS1所需的区域被映射到具有残留物R38和K41的蛋白质的RNA结合氨基末端基序对于活性至关重要。虽然我们表明NS1可以直接绑定到MRNA，但它与其刺激翻译的能力不相关。这种活动相当依赖于NS1的性质与核糖体相关，并招募它们以靶向MRNA。图形抽象显示省略了亮点？ NS1增强了发起水平的翻译。还是NS1与MRNA和核糖体结合。还是仅需要NS1对核糖体的结合来进行翻译刺激。

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