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首页> 外文期刊>Journal of Molecular Biology >The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei
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The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei

机译：规范聚（a）聚合酶PAP1多腺苷酸非编码RNA，对于锥虫瘤Brucei中的Snorna生物生物是必不可少的

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Abstract The parasite Trypanosoma brucei is the causative agent of African sleeping sickness and is known for its unique RNA processing mechanisms that are common to all the kinetoplastidea including Leishmania and Trypanosoma cruzi. Trypanosomes possess two canonical RNA poly (A) polymerases (PAPs) termed PAP1 and PAP2. PAP1 is encoded by one of the only two genes harboring cis -spliced introns in this organism, and its function is currently unknown. In trypanosomes, all mRNAs, and non-coding RNAs such as small nucleolar RNAs (snoRNAs) and long non-coding RNAs (lncRNAs), undergo trans- splicing and polyadenylation. Here, we show that the function of PAP1, which is located in the nucleus, is to polyadenylate non-coding RNAs, which undergo trans- splicing and polyadenylation. Major substrates of PAP1 are the snoRNAs and lncRNAs. Under the silencing of either PAP1 or PAP2, the level of snoRNAs is reduced. The dual polyadenylation of snoRNA intermediates is carried out by both PAP2 and PAP1 and requires the factors essential for the polyadenylation of mRNAs. The dual polyadenylation of the precursor snoRNAs by PAPs may function to recruit the machinery essential for snoRNA processing. Highlights ? PAP1 is encoded by one of the two genes in trypanosomes containing cis -intron. ? snoRNA precursors and lncRNAs are polyadenylated by two PAPs, PAP1 and PAP2. ? snoRNA polyadenylation requires the canonical polyadenylation machinery. ? snoRNA processing requires PAP1 or polyadenylation by PAP1. Graphical Abstract Display Omitted
机译：摘要寄生虫胰蛋白酶瘤Brucei是非洲睡眠疾病的致病因子，并且以其独特的RNA加工机制而闻名，这些机制是所有Kinetoplastidea，包括Leishmania和Trypanosoma Cruzi。锥虫具有两个规范RNA聚（A）聚合酶（PAPS）称为PAP1和PAP2。 PAP1由在该生物体中含有CIS-CIS型内含子的唯一两个基因编码，其功能目前未知。在锥虫中，所有MRNA和非编码RNA，如小核仁RNA（SNARNAS）和长的非编码RNA（LNCRNA），经历转削和多腺苷酸。在这里，我们表明，位于核中的PAP1的功能是聚酰基化非编码RNA，其经历转削和多腺苷酸化。 PAP1的主要基板是Snornas和LNCRNA。在PAP1或PAP2的沉默下，减少了Snornas的水平。 Snorna中间体的双聚腺苷酸由PAP2和PAP1进行，并且需要MRNA的多腺苷酸化必需的因素。 PAPS的前体护腿的双聚腺苷酸可以起作用以招募用于护垫加工的机械。强调 ？ PAP1由含有CIS -INTRON的锥虫中的两个基因中的一个编码。还是Snorna前体和LNCRNA由两个PAPS，PAP1和PAP2是多腺苷酸化。还是Snorna多腺苷酸化需要典型的多腺苷酸化机械。还是护腿处理需要PAP1或多腺苷酸。省略了图形抽象显示

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