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首页> 外文期刊>Journal of Neurophysiology >Optogenetic manipulation of medullary neurons in the locust optic lobe
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Optogenetic manipulation of medullary neurons in the locust optic lobe

机译：尖塔髓质神经元的致敏操纵

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摘要

The locust is a widely used animal model for studying sensory processing and its relation to behavior. Due to the lack of genomic information, genetic tools to manipulate neural circuits in locusts are not yet available. We examined whether Semliki Forest virus is suitable to mediate exogenous gene expression in neurons of the locust optic lobe. We subcloned a channelrhodopsin variant and the yellow fluorescent protein Venus into a Semliki Forest virus vector and injected the virus into the optic lobe of locusts (Schistocerca americana). Fluorescence was observed in all injected optic lobes. Most neurons that expressed the recombinant proteins were located in the first two neuropils of the optic lobe, the lamina and medulla. Extracellular recordings demonstrated that laser illumination increased the firing rate of medullary neurons expressing channelrhodopsin. The optogenetic activation of the medullary neurons also triggered excitatory postsynaptic potentials and firing of a postsynaptic, looming-sensitive neuron, the lobula giant movement detector. These results indicate that Semliki Forest virus is efficient at mediating transient exogenous gene expression and provides a tool to manipulate neural circuits in the locust nervous system and likely other insects.
机译：蝗虫是一种广泛使用的动物模型，用于研究感官处理及其与行为的关系。由于缺乏基因组信息，遗传工具可以在蝗虫中操纵神经电路。我们检查了Semliki林病毒是否适合在蝗虫视神经的神经元中介导外源基因表达。我们将渠道式素变体和黄色荧光蛋白Venus亚克隆到Semliki林病毒载体中，并将​​病毒注入蝗虫（Schistocerca Americana）的视神经中。在所有注入的光瓣中观察到荧光。大多数表达重组蛋白质的神经元位于视神经叶，椎板和髓质的前两个神经石。细胞外记录表明，激光照射增加了表达肠系中髓质神经元的烧制率。髓质神经元的致敏活化也引发了兴奋性的突触后潜在和射击后突触，狭窄敏感的神经元，叶片巨型运动探测器。这些结果表明，Semliki林病毒在介导短暂的外源基因表达上是有效的，并提供一种工具，以操纵蝗虫神经系统中的神经电路和其他昆虫。

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