A novel low-signal image enhancement method for multiphoton microscopy

Lin Hongxin; Wei Chao; Cao Ning; Chen Hu; Wang Guangxing; Chen Jianxin; Chen Guannan; Zhuo Shuangmu

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A novel low-signal image enhancement method for multiphoton microscopy

机译：一种用于多光子显微镜的新型低信号图像增强方法

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摘要

With the expansion of multiphoton microscopy (MPM) applications in biomedical fields, imaging quality improvement remains a challenge. In this paper, a novel low-signal image enhancement method for MPM (LSIE-MPM) is presented that combines chi-squared conversion function and brightness transform function models. The proposed method has been applied to MPM imaging for the first time, including plant cell imaging, fresh ovary issue imaging, and real-time video improvement. Experimental results show that compared to other state-of-the-art algorithms, LSIE-MPM has superior accuracy and robustness.

机译：随着Multiphoton显微镜（MPM）应用在生物医学领域的应用，成像质量改善仍然是一个挑战。本文提出了一种用于MPM（LSIE-MPM）的新型低信号图像增强方法，其结合了CHI方向转换函数和亮度变换功能模型。该方法已首次应用于MPM成像，包括植物细胞成像，新鲜卵巢问题成像和实时视频改进。实验结果表明，与其他最先进的算法相比，LSIE-MPM具有卓越的精度和鲁棒性。

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来源
《Journal of Physics, D. Applied Physics: A Europhysics Journal》 |2019年第28期|共10页
作者
Lin Hongxin; Wei Chao; Cao Ning; Chen Hu; Wang Guangxing; Chen Jianxin; Chen Guannan; Zhuo Shuangmu;
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作者单位

Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Fuzhou Fujian Peoples R China;

Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Fuzhou Fujian Peoples R China;

Fujian Med Univ Dept Plast Surg Zhangzhou Affiliated Hosp Zhangzhou Peoples R China;

Fujian Med Univ Union Hosp Fuzhou Fujian Peoples R China;

Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Fuzhou Fujian Peoples R China;

Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Fuzhou Fujian Peoples R China;

Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Fuzhou Fujian Peoples R China;

Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Fuzhou Fujian Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类应用物理学;
关键词
multiphoton microscopy (MPM); low-signal image enhancement; chi-squared conversion function (CCF); brightness transform function (BTF);

机译：多光子显微镜（MPM）;低信号图像增强;CHI方向转换功能（CCF）;亮度变换功能（BTF）;

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专利

1. A novel low-signal image enhancement method for multiphoton microscopy [J] . Lin Hongxin, Wei Chao, Cao Ning, Journal of Physics, D. Applied Physics: A Europhysics Journal . 2019,第28期

机译：一种用于多光子显微镜的新型低信号图像增强方法
2. Discussion of methods for depth enhancement in single and multiphoton-stimulated emission microscopy [J] . Frankel Robert D. Journal of the Optical Society of America, B. Optical Physics . 2016,第7期

机译：对单光子和多光子激发发射显微镜中的深度增强方法的讨论
3. Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo [J] . Camilla A. Thorling Yuri Dancik Clinton W. Hupple Gregory Medley Xin Liu Andrei V. Zvyagin Tom A. Robertson Frank J. Burczynski and Michael S. Roberts journal of biomedical optics . 2011,第8期

机译：多光子显微镜和荧光寿命成像为研究大鼠肝脏体内药物分布和代谢提供了一种新方法
4. Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy [C] . Dongeun Kim, Wonsang Hwang, Youngjae Won, Conference on multiphoton microscopy in the biomedical sciences . 2018

机译：提高多光子检测荧光寿命成像显微镜的测量速度和光子经济性
5. The Integration of Computational Methods and Nonlinear Multiphoton Multimodal Microscopy Imaging for the Analysis of Unstained Human and Animal Tissues [D] . Murashova, Gabrielle Alyse. 2019

机译：计算方法的整合和非线性多光子多模式显微镜成像分析未染色人和动物组织
6. A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines [O] . Charlotte Bureau, Nadège Lanau, Mathieu Ingouff, 2018

机译：结合多光子显微镜和碘化丙啶进行水稻深3D根分生组织成像的协议：在筛选和鉴定组织特异性增强子陷阱系中的应用
7. Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo [O] . Thorling, Camilla A, Dancik, Yuri, Hupple, Clinton W, 2011

机译：多光子显微镜和荧光寿命成像为研究大鼠肝脏体内药物分布和代谢提供了一种新方法

A novel low-signal image enhancement method for multiphoton microscopy

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