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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >A novel surface plasmon resonance biosensor based on graphene oxide decorated with gold nanorod-antibody conjugates for determination of transferrin
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A novel surface plasmon resonance biosensor based on graphene oxide decorated with gold nanorod-antibody conjugates for determination of transferrin

机译：基于金纳米棒-抗体结合物修饰的氧化石墨烯的表面等离子体共振生物传感器测定转铁蛋白

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摘要

A surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) decorated with gold nanorod (AuNR)-antibody conjugates was developed. Compared with traditional SPR biosensor, GO sheets were assembled on the amino-modified Au film via electrostatic interaction, and then AuNR-antibody conjugates were immobilized via carbodiimide-assisted amidation reaction. The abundant oxygen-containing functional groups, large specific surface area and friendly biocompatibility of GO sheets are beneficial to the immobilization of AuNR-antibody conjugates. Meanwhile, AuNRs are anchored to the GO surface through antibody and function as enhancers for the transferrin determination, which greatly enhance the sensitivity of the detection. As a result, the present biosensor shows a satisfactory response to transferrin in the concentration range of 0.0375-40.00μgmL~(-1). The lowest concentration of transferrin that can be determined by this method is about 32-fold lower than that obtained with the sensor based on Au film. This design affords a facile method of controlling the assembly of AuNRs on the GO sheets and can be easily extended to other protein detection by preparing corresponding AuNR-antibody conjugates.
机译：基于金纳米棒（AuNR）-抗体缀合物修饰的基于氧化石墨烯（GO）的表面等离子体共振（SPR）生物传感器被开发出来。与传统的SPR生物传感器相比，GO片材通过静电相互作用组装在氨基修饰的Au膜上，然后通过碳二亚胺辅助的酰胺化反应固定AuNR-抗体结合物。 GO薄层具有丰富的含氧官能团，大的比表面积和友好的生物相容性，有利于AuNR-抗体结合物的固定。同时，AuNRs通过抗体锚定在GO表面，并作为转铁蛋白测定的增强剂，极大地提高了检测的灵敏度。结果，本发明的生物传感器在0.0375-40.00μgmL〜（-1）的浓度范围内对转铁蛋白表现出令人满意的响应。与基于金膜的传感器相比，可以通过这种方法确定的最低转铁蛋白浓度约低32倍。该设计提供了一种控制GO板上AuNRs组装的简便方法，并且可以通过制备相应的AuNR-抗体偶联物轻松地扩展到其他蛋白质检测。

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