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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach
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A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach

机译：使用基于量子点的FRET方法进行有创和无创NSCLC早期检测的启动子甲基化标记物组

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摘要

Non-small-cell lung cancer (NSCLC) leads to a significant proportion of cancer-related deaths, and early detection of NSCLC can significantly increase cancer survival rates. A promising approach has been studied to exploit DNA methylation, which is closely correlated to early cancer diagnosis. Herein, in order to realize the early detection of NSCLC, we utilized the developed quantum dots-based (QDs-based) fluorescence resonance energy transfer (FRET) nanosensor technique to analyze the promoter methylation in early stage NSCLC tissue samples and noninvasive bronchial brushing specimens. Using this method, the methylation levels can be quantitatively determined by measuring the signal amplification during FRET. A panel of three tumor suppressor genes (PCDHGB6, HOXA9 and RASSF1A) was assessed in 50 paired early stage NSCLC and their adjacent nontumorous tissue (NT) samples, and 50 early stage NSCLC bronchial brushing and normal specimens. The combined detection was able to identify not only tissue samples but noninvasive bronchial brushing specimens from control cases with a high degree of sensitivity of 92% (AUC=0.977, P < 0.001) and 80% (AUC=0.907, P < 0.001) respectively, indicating the versatility of promoter expression in invasive and noninvasive NSCLC samples. Therefore this approach can be used to sensitively analyze the methylation levels of cancer-related genes, which might be a potential tool for noninvasive early clinical diagnosis of cancers. (C) 2016 Elsevier B.V. All rights reserved.
机译：非小细胞肺癌（NSCLC）导致相当一部分与癌症相关的死亡，并且早期发现NSCLC可以显着提高癌症的存活率。已经研究了一种有前途的方法来利用DNA甲基化，这与早期癌症诊断密切相关。在此，为了实现对NSCLC的早期检测，我们利用已开发的基于量子点（基于QDs）的荧光共振能量转移（FRET）纳米传感器技术来分析早期NSCLC组织样本和非侵入性支气管刷洗样本中的启动子甲基化。使用这种方法，可以通过测量FRET期间的信号放大来定量确定甲基化水平。在50个配对的早期NSCLC及其相邻的非肿瘤组织（NT）样本以及50个早期NSCLC早期支气管刷和正常样本中评估了一组三个抑癌基因（PCDHGB6，HOXA9和RASSF1A）。组合检测不仅能够识别组织样本，而且能够识别对照组的非侵入性支气管刷洗样本，其敏感度分别为92％（AUC = 0.977，P <0.001）和80％（AUC = 0.907，P <0.001） ，表明在有创和无创NSCLC样品中启动子表达的多功能性。因此，该方法可用于灵敏地分析癌症相关基因的甲基化水平，这可能是癌症非侵入性早期临床诊断的潜在工具。 （C）2016 Elsevier B.V.保留所有权利。

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