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Light reaches the very heart of the zebrafish clock

机译:光线直射斑马鱼钟的心脏

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摘要

Zebrafish are typically used as a model system to study various aspects of developmental biology, largely as a consequence of their ex vivo development, high degree of transparency, and, of course, ability to perform forward genetic mutant screens. More recently, zebrafish have been developed as a model system with which to study circadian clocks. Cell lines generated from early-stage zebrafish embryos contain clocks that are directly light-responsive. We describe recent experiments using single-cell luminescent imaging approaches to study clock function in this novel cell line system. Furthermore, studies examining the process of entrainment to light pulses within this cell population are described in this review, as are experiments examining light-responsiveness of early-stage zebrafish embryos.
机译:斑马鱼通常用作模型系统来研究发育生物学的各个方面,这主要是由于它们的离体发育,高度的透明性以及进行正向遗传突变体筛选的能力。最近,斑马鱼已被开发为用于研究生物钟的模型系统。早期斑马鱼胚胎产生的细胞系包含直接对光反应的时钟。我们描述了使用单细胞发光成像方法的最新实验,以研究这种新型细胞系系统中的时钟功能。此外,在这篇综述中描述了研究对该细胞群内的光脉冲进行夹带的研究,以及检验早期斑马鱼胚胎的光响应性的实验。

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