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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Constructing a novel 8-hydroxy-2 '-deoxyguanosine electrochemical sensor and application in evaluating the oxidative damages of DNA and guanine
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Constructing a novel 8-hydroxy-2 '-deoxyguanosine electrochemical sensor and application in evaluating the oxidative damages of DNA and guanine

机译：新型8-羟基-2'-脱氧鸟苷电化学传感器的构建及其在DNA和鸟嘌呤氧化损伤评价中的应用

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摘要

8-Hydroxy-2'-deoxyguanosine (8-OHdG) is commonly identified as a biomarker of oxidative DNA damage. In this work, a novel and facile 8-OHdG sensor was developed based on the multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode (GCE). It exhibited good electrochemical responses toward the oxidation of 8-OHdG, and the linear ranges were 5.63 x 10(-8) - 6.08 x 10(-6) M and 6.08 x 10(-6) - 1.64 x 10(-5) M, with the detection limit of 1.88 x 10(-8) M (S/N=3). Moreover, the fabricated sensor was applied for the determination of 8-OHdG generated from damaged DNA and guanine, respectively, and the oxidation currents of 8-OHdG increased along with the damaged DNA and guanine within certain concentrations. These results could be used to evaluate the DNA damage, and provide useful information on diagnosing diseases caused by mutation and deficiency of the immunity system. (C) 2016 Elsevier B.V. All rights reserved.
机译：通常将8-Hydroxy-2'-deoxyguanosine（8-OHdG）鉴定为氧化DNA损伤的生物标记。在这项工作中，基于多壁碳纳米管（MWCNTs）修饰的玻璃碳电极（GCE），开发了一种新颖且方便的8-OHdG传感器。它对8-OHdG的氧化表现出良好的电化学反应，线性范围为5.63 x 10（-8）-6.08 x 10（-6）M和6.08 x 10（-6）-1.64 x 10（-5） M，检测限为1.88 x 10（-8）M（S / N = 3）。此外，将所制造的传感器分别用于测定受损DNA和鸟嘌呤产生的8-OHdG，并且在一定浓度下8-OHdG的氧化电流随受损DNA和鸟嘌呤的增加而增加。这些结果可用于评估DNA损伤，并为诊断由免疫系统突变和缺乏引起的疾病提供有用的信息。 （C）2016 Elsevier B.V.保留所有权利。

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