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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods
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Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods

机译：用全内反射椭圆光度法和QCM阻抗法记录T-2霉菌毒素

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摘要

A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 mu g/mI down to 0.15 ng/ml. Association constants of 1.4 x 10(6) and 1.9 x 10(7) mol(-1) s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4-5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies. (c) 2006 Elsevier B.V. All rights reserved.
机译：利用全内反射椭圆光度法（TIRE）和免疫测定方法的灵敏光学方法，可以在从100μg / mI到0.15 ng / ml的广泛浓度范围内记录T-2霉菌毒素。从TIRE动力学测量评估分别为多和单克隆T-2抗体的1.4 x 10（6）和1.9 x 10（7）mol（-1）s的缔合常数。根据TIRE数据拟合，T-2分子与抗体的结合（处于饱和状态）导致吸附层厚度增加4-5 nm。 QCM阻抗测量数据显示异常大的质量增加和膜软化，这很可能是由于大的T-2聚集体与抗体结合所致。 （c）2006 Elsevier B.V.保留所有权利。

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