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首页> 外文期刊>Academic radiology >Magnetic resonance imaging of mesenchymal stem cells labeled with dual (MR and fluorescence) agents in rat spinal cord injury.
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Magnetic resonance imaging of mesenchymal stem cells labeled with dual (MR and fluorescence) agents in rat spinal cord injury.

机译：在大鼠脊髓损伤中用双重（MR和荧光）试剂标记的间充质干细胞的磁共振成像。

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RATIONALE AND OBJECTIVES: In vivo tracking cells using gadolinium-based contrast agents have the important advantage of providing a positive contrast on T1-weighted images, which is less likely to be confused with artifacts because of postoperative local signal voids such as metal, hemorrhage, or air. The aim of this study is to paramagnetically and fluorescently label marrow with dual agents (gadolinium-diethylene triamine penta-acetic acid [Gd-DTPA] and PEI-FluoR) and track them after transplantation into spinal cord injury (SCI) with magnetic resonance imaging (MRI). MATERIALS AND METHODS: Marrow mesenchymal stem cells (MSCs) from Sprague-Dawley rats were incubated with PEI-FluoR (rhodamine-conjugated PEI-FluoR) and Gd-DTPA complex for labeling. After labeling, cellular viability, proliferation, and apoptosis were evaluated. T1 value and longevity of intracellular Gd-DTPA retention were measured on a 1.5 T MRI scanner. Thirty-six SCI rats were implanted with labeled and unlabeled MSCs and phosphate-buffered saline. Then, serial MRI and Basso-Beattie-Bresnehan (BBB) locomotor tests were performed and correlated with fluorescent microscopy. The relative signal intensity (RSL) of the engraftment in relation to normal cord was measured and the linear mixed model followed by post-hoc Bonferroni test was used to identify significant differences in RSL as well as BBB score. RESULTS: MSCs could be paramagnetically and fluorescently labeled by the dual agents. The labeling did not influence the cellular viability, proliferation, and apoptosis. The longevity of Gd-DTPA retention in labeled MSCs was up to 21 days. The distribution and migration of labeled MSCs in SCI lesions could be tracked until 7 days after implantation on MRI. The relative signal intensities of SCI rats treated with labeled cells at 1 day and 3 days (1.34 +/- 0.02, 1.27 +/- 0.03) were significantly higher than rats treated with unlabeled cells (0.94 +/- 0.01, 0.99 +/- 0.02) and phosphate-buffered saline (0.91 +/- 0.01, 0.95 +/- 0.01) (P < .05). Rats treated with labeled MSCs or unlabeled MSCs achieved significantly higher BBB scores than controls at 14, 21, 28, and 35 days after injury (P < .05). CONCLUSIONS: Labeling MSCs with the dual agents may enable cellular MRI and tracking in experimental spinal cord injury.
机译：理由和目的：使用基于lin的造影剂的体内追踪细胞具有在T1加权图像上提供正对比度的重要优势，由于术后局部信号空隙（例如金属，出血，或空气。这项研究的目的是用双重试剂（ga-二亚乙基三胺五乙酸[Gd-DTPA]和PEI-FluoR）对骨髓进行顺磁性和荧光标记，并通过磁共振成像对它们植入脊髓损伤（SCI）后进行追踪（MRI）。材料与方法：将Sprague-Dawley大鼠的骨髓间充质干细胞（PEs）与PEI-FluoR（若丹明缀合的PEI-FluoR）和Gd-DTPA复合物孵育，进行标记。标记后，评估细胞活力，增殖和凋亡。在1.5 T MRI扫描仪上测量T1值和细胞内Gd-DTPA保留的寿命。将36只SCI大鼠植入标记和未标记的MSC和磷酸盐缓冲液。然后，进行了串行MRI和Basso-Beattie-Bresnehan（BBB）运动测试，并将其与荧光显微镜相关。测量相对于正常脐带的植入物的相对信号强度（RSL），并使用线性混合模型，然后进行事后Bonferroni检验，以鉴定RSL和BBB评分的显着差异。结果：MSCs可以被双重试剂顺磁性和荧光标记。标记不影响细胞活力，增殖和凋亡。 Gd-DTPA在标记的MSC中的保留时间长达21天。可以追踪标记的MSC在SCI病变中的分布和迁移，直到MRI植入后7天为止。在第1天和第3天用标记细胞治疗的SCI大鼠的相对信号强度（1.34 +/- 0.02，1.27 +/- 0.03）显着高于未标记细胞治疗的大鼠（0.94 +/- 0.01，0.99 +/-） 0.02）和磷酸盐缓冲盐水（0.91 +/- 0.01，0.95 +/- 0.01）（P <.05）。在损伤后第14、21、28和35天，用标记的MSC或未标记的MSC治疗的大鼠的BBB得分明显高于对照组（P <.05）。结论：用双重药剂标记MSCs可以使细胞MRI和跟踪实验性脊髓损伤。

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