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首页> 外文期刊>Acta Agronomica Hungarica >ESTIMATION OF POLLEN VIABILITY IN EINKORN (TRITICUM MONOCOCCUM SSP. MONOCOCCUM) ACCESSIONS OF DIFFERENT GEOGRAPHICAL ORIGIN
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ESTIMATION OF POLLEN VIABILITY IN EINKORN (TRITICUM MONOCOCCUM SSP. MONOCOCCUM) ACCESSIONS OF DIFFERENT GEOGRAPHICAL ORIGIN

机译:不同地理来源的艾因科恩粉(粉状小麦)的花粉生活力估计

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摘要

In the present experiment the pollen viability of einkorn accessions of different origin was tested using four vital dyes (TTC, Baker's procedure, MTT and FDA) to determine the potential of the dyes to differentiate fresh living pollen from pollen heated for 12 hours at 80°C (killed pollen). It was found that two of the four dyes previously employed to determine pollen viability also stained killed pollen in the case of several einkorn accessions, while FDA and MTT did not. It is thus suggested thatthe two latter should be used to test einkorn pollen viability, since they do not normally stain either killed or aborted pollen grains.
机译:在本实验中,使用四种重要染料(TTC,Baker's程序,MTT和FDA)测试了不同来源的Einkorn品种的花粉生存力,以确定该染料将新鲜活花粉与80°C加热12小时的花粉区分开来的潜力。 C(花粉杀死)。我们发现,以前用于确定花粉生存力的四种染料中的两种,在几种Einkorn保藏号的情况下,也能杀死被杀死的花粉,而FDA和MTT则没有。因此,建议后者应用于测试单粒花粉的生存力,因为它们通常不染色或杀死花粉粒。

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