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首页> 外文期刊>Acta biomaterialia >Matrix stiffness affects spontaneous contraction of cardiomyocytes cultured within a PEGylated fibrinogen biomaterial.
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Matrix stiffness affects spontaneous contraction of cardiomyocytes cultured within a PEGylated fibrinogen biomaterial.

机译：基质刚度影响在聚乙二醇化的纤维蛋白原生物材料中培养的心肌细胞的自发收缩。

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摘要

Successful implementation of cardiac cell transplantation for treating damaged myocardium relies on the development of improved injectable biomaterials. A novel biomaterial technology using PEGylated fibrinogen has been developed with controllable physicochemical properties based on the poly(ethylene glycol) (PEG) constituent. In addition, the fibrinogen backbone of the material confers inherent bioactivity to cells. The purpose of this investigation was to explore by in vitro techniques the use of this biomaterial as a scaffold for cardiac tissue regeneration. To this end neonatal rat cardiomyocytes were cultivated in PEGylated fibrinogen constructs. The cell-seeding density and biomaterial composition were optimized to obtain maximum spontaneous contraction of the constructs. Quantitative characterization of the contraction pattern was accomplished by video image analysis. It was possible to demonstrate an inverse correlation between the material stiffness and the amplitude of contraction of the tissue constructs by changing the modulus of the matrix using different compositions of PEG and fibrinogen. The relationship between matrix stiffness, cell density and tissue contraction also provided some insight into the mechanism of cellular remodeling that ultimately leads to synchronized contraction of the constructs. These findings indicate that PEGylated fibrinogen hydrogels can be used as a scaffold for cardiomyocytes, and offer the possibility of controlling cellular remodeling via simple compositional modifications to the matrix.
机译：心脏细胞移植治疗受损心肌的成功实施取决于改进的可注射生物材料的开发。基于聚（乙二醇）（PEG）成分，已开发出一种具有可控物理化学性质的，使用聚乙二醇化纤维蛋白原的新型生物材料技术。另外，该材料的纤维蛋白原主链赋予细胞固有的生物活性。这项研究的目的是通过体外技术探索这种生物材料作为心脏组织再生支架的用途。为此，将新生大鼠心肌细胞培养在PEG化的纤维蛋白原构建体中。优化细胞播种密度和生物材料组成，以使构建体获得最大的自发收缩。通过视频图像分析完成了收缩模式的定量表征。通过使用不同的PEG和纤维蛋白原成分改变基质的模量，有可能证明材料刚度与组织构建体收缩幅度之间呈负相关。基质刚度，细胞密度和组织收缩之间的关系也提供了对细胞重构机制的一些见解，该机制最终导致构建体的同步收缩。这些发现表明，聚乙二醇化的纤维蛋白原水凝胶可用作心肌细胞的支架，并提供了通过对基质的简单组成修饰来控制细胞重塑的可能性。

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