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首页> 外文期刊>Acta biomaterialia >Macrophage response to methacrylate conversion using a gradient approach.
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Macrophage response to methacrylate conversion using a gradient approach.

机译：巨噬细胞对使用梯度方法的甲基丙烯酸酯转化的响应。

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摘要

Incomplete conversion, an ongoing challenge facing photopolymerized methacrylate-based polymers, affects leachables as well as the resulting polymer network. As novel polymers and composites are developed, methods to efficiently screen cell response to these materials and their properties, including conversion, are needed. In this study, an in vitro screening methodology was developed to assess cells cultured directly on cross-linked polymer networks. A gradient in methacrylate double bond conversion was used to increase the experimental throughput. A substrate of 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenyl] propane (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) was prepared with a conversion ranging from 43.0% to 61.2%. Substrates aged for 7 days had no significant differences in surface roughness or hydrophilicity as a function of conversion. Leachables were detectable for at least 7 days using UV absorption, but their global cytotoxicity was insignificant after 5 days of aging. Thus, RAW 264.7 macrophage-like cells were cultured on aged substrates to evaluate the cell response to conversion, with possible contributions from the polymer network and local leachables. Conversions of 45% and 50% decreased viability (via calcein/ethidium staining) and increased apoptosis (via annexin-V staining). No significant changes (p>0.05) in tumor necrosis factor-alpha and interleukin-1beta gene expression, as measured by quantitative, real-time reverse transcription-polymerase chain reaction, were seen as conversion increased. Thus, conversions greater than 50% are recommended for equimolar BisGMA/TEGDMA. The ability to distinguish cell response as a function of conversion is useful as an initial biological screening platform to optimize dental polymers.
机译：不完全转化是光聚合的基于甲基丙烯酸酯的聚合物面临的挑战，它会影响可浸出物以及最终的聚合物网络。随着新型聚合物和复合材料的开发，需要有效筛选细胞对这些材料及其特性（包括转化）的反应的方法。在这项研究中，开发了一种体外筛选方法来评估直接在交联的聚合物网络上培养的细胞。使用甲基丙烯酸酯双键转化率的梯度来提高实验通量。制备2，2-双[4-（2-羟基-3-甲基丙烯酰氧基丙氧基）苯基]丙烷（BisGMA）和三甘醇二甲基丙烯酸酯（TEGDMA）的底物，其转化率为43.0％至61.2％。老化7天的基材在表面粗糙度或亲水性与转化率之间没有显着差异。使用紫外线吸收，可浸出物至少可检测7天，但老化5天后其整体细胞毒性微不足道。因此，在老化的基质上培养RAW 264.7巨噬细胞样细胞，以评估细胞对转化的反应，其中可能来自聚合物网络和局部浸出物。 45％和50％的转化率降低了生存能力（通过钙黄绿素/乙锭染色），并增加了细胞凋亡（通过Annexin-V染色）。通过定量实时逆转录-聚合酶链反应测量，肿瘤坏死因子-α和白细胞介素-1β基因表达无明显变化（p> 0.05），观察到转化率增加。因此，对于等摩尔的BisGMA / TEGDMA，建议转化率大于50％。区分细胞响应与转化的关系的能力可用作优化生物聚合物的初始生物学筛选平台。

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