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首页> 外文期刊>Acta biomaterialia >Analysis of functionalized polyethylene terephthalate with immobilized NTPDase and cysteine.
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Analysis of functionalized polyethylene terephthalate with immobilized NTPDase and cysteine.

机译：固定化NTPDase和半胱氨酸对功能化聚对苯二甲酸乙二酯的分析。

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摘要

Polyethylene terephthalate (PET) was functionalized to introduce carboxyl groups onto its surface by a carboxylation technique. Surface and bulk properties, such as possible surface deterioration, surface roughness and the mechanical strength of the carboxylated polymers, were studied and compared with those of aminolyzed and hydrolyzed PET. Atomic force microscopy studies showed that unlike aminolysis and hydrolysis, which increased the surface roughness significantly due to cracking and pitting, the surface roughness of unmodified and carboxylated PET were comparable. While hydrolysis and aminolysis of PET resulted in significant loss of strength, tensile testing revealed that unmodified and carboxylated polymers had similar strength. The development of mechanically stable, functionalized PET would vastly improve the biomedical applications of this polymer. To understand the potential for improving biomedical applications, biologically active molecules, namely nucleoside triphosphate diphosphohydrolase (NTPDase) and cysteine, were immobilized on the carboxylated PET using amide bonds. NTPDase was also immobilized to aminolyzed PET using imine bonds, while cysteine was immobilized on aminolyzed PET using both imine and amide bonds. Attachment of NTPDase and cysteine was verified by analyzing the NTPDase activity and the cysteine surface concentration. The stability of these immobilizations was also studied.
机译：通过羧化技术对聚对苯二甲酸乙二酯（PET）进行功能化以将羧基引入其表面。研究了表面和整体性质，例如可能的表面变质，表面粗糙度和羧化聚合物的机械强度，并与氨基水解和水解的PET进行了比较。原子力显微镜研究表明，与氨解和水解不同，由于裂化和点蚀，氨水解和水解显着增加了表面粗糙度，未改性和羧化PET的表面粗糙度相当。尽管PET的水解和氨解会导致强度显着下降，但拉伸试验表明，未改性和羧化的聚合物具有相似的强度。机械稳定，功能化的PET的发展将极大地改善这种聚合物的生物医学应用。为了了解改善生物医学应用的潜力，使用酰胺键将生物活性分子，即核苷三磷酸二磷酸水解酶（NTPDase）和半胱氨酸固定在羧化PET上。还使用亚胺键将NTPDase固定在氨基裂解的PET上，同时使用亚胺和酰胺键将半胱氨酸固定在氨基裂解的PET上。通过分析NTPDase活性和半胱氨酸表面浓度来验证NTPDase和半胱氨酸的附着。还研究了这些固定化的稳定性。

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