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首页> 外文期刊>Acta biomaterialia >Using self-assembled monolayers to model the extracellular matrix.
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Using self-assembled monolayers to model the extracellular matrix.

机译：使用自组装单分子层对细胞外基质进行建模。

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摘要

The extracellular matrix is an insoluble aggregate of large proteins and glycosoaminoglycans that comprises the microenvironment of cells in tissue. The matrix displays a host of ligands that interact with cell-surface receptors to mediate the attachment and spreading of cells and regulate signaling processes. Studies of cell-matrix interactions and downstream signaling processes commonly employ substrates having an adsorbed layer of protein and are challenged by the difficulty in controlling the structure and activity of the immobilized protein. Significant effort has been directed towards the development of model substrates that present adhesion ligands in defined densities, orientations and environments. Among these approaches, self-assembled monolayers of alkanethiolates on gold offer a high level of control over the molecular structure of the surface and are well-suited to studies of cell adhesion. This review describes the design and use of monolayers for applications in cell biology, including the use of monolayers to evaluate the roles of peptide and protein ligands in cell-matrix interactions, the development of methods to pattern ligands on monolayers and applications to cell biology, the development of dynamic monolayers that can switch the activities of ligands presented to an adherent cell, and the rewiring of interactions between a cell and its substrate. These examples illustrate the flexibility inherent to monolayers for applications in cell biology.
机译：细胞外基质是大蛋白质和糖胺聚糖的不溶性聚集体，其构成组织中细胞的微环境。基质显示出许多与细胞表面受体相互作用的配体，以介导细胞的附着和扩散并调节信号传导过程。细胞-基质相互作用和下游信号传导过程的研究通常使用具有蛋白质吸附层的底物，并且难以控制固定的蛋白质的结构和活性。已经进行了巨大的努力以开发在限定的密度，取向和环境下呈现粘合配体的模型基材。在这些方法中，链烷硫醇盐在金上的自组装单层提供了对表面分子结构的高度控制，非常适合于细胞粘附的研究。这篇综述描述了在细胞生物学中应用的单层膜的设计和使用，包括使用单层膜评估肽和蛋白质配体在细胞-基质相互作用中的作用，在单层膜上构图配体的方法的开发以及在细胞生物学中的应用，动态单层的发展，可以改变呈现给粘附细胞的配体的活性，以及​​重新布线细胞与其底物之间的相互作用。这些例子说明了单层固有的灵活性，可用于细胞生物学。

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