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首页> 外文期刊>Acta biomaterialia >Vascularization of wide pore agarose-gelatin cryogel scaffolds implanted subcutaneously in diabetic and non-diabetic mice.
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Vascularization of wide pore agarose-gelatin cryogel scaffolds implanted subcutaneously in diabetic and non-diabetic mice.

机译：在糖尿病和非糖尿病小鼠中皮下植入的宽孔琼脂糖-明胶冷冻凝胶支架的血管化。

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Polymeric scaffolds have been reported to promote angiogenesis, facilitating oxygen delivery; however, little is known about the effect of diabetes on the neo-vascularization of implanted polymeric scaffolds at subcutaneous (SC) sites. In this study we compare the effect of diabetes on scaffold vascularization following SC implantation into diabetic and non-diabetic mice. Wide pore agarose cryogel scaffolds with grafted gelatin were prepared by a two-step freezing procedure and subsequent thawing. The scaffolds were implanted subcutaneously into streptozoticin-induced diabetic mice and control, non-diabetic mice. The vascularization process was estimated using histological sections, in which endothelial cells were identified by Von Willebrand factor (vWF) and CD31 antigen staining and the pericyte layer was confirmed by alpha-smooth muscle actin (alpha-SMA) visualization. Comparative analysis showed a similar thickness of fibrous capsules around the vascularized scaffolds in both diabetic and non-diabetic animals. Intensive staining for alpha-SMA indicated the formation of mature blood vessels in the surrounding fibrous capsule and tissue invading the scaffold area. No statistically significant differences in capillary density and area occupied by blood vessels were found between diabetic and non-diabetic mice. In conclusion, the present study shows no adverse effects of diabetes on new blood vessel formation in SC implanted agarose cryogel scaffolds with grafted gelatin.
机译：据报道，聚合物支架可促进血管生成，促进氧的输送。然而，关于糖尿病对皮下（SC）部位植入的聚合物支架新血管形成的影响知之甚少。在这项研究中，我们比较了糖尿病对SC植入糖尿病和非糖尿病小鼠后支架血管生成的影响。通过两步冷冻程序并随后解冻，制备具有接枝明胶的宽孔琼脂糖冷冻凝胶支架。将支架皮下植入链脲霉素诱导的糖尿病小鼠和非糖尿病对照小鼠中。使用组织学切片估计血管形成过程，其中通过冯威勒布兰德因子（vWF）和CD31抗原染色鉴定内皮细胞，并通过α平滑肌肌动蛋白（α-SMA）可视化确认周细胞层。对比分析显示，在糖尿病动物和非糖尿病动物中，血管化支架周围的纤维囊厚度相似。对α-SMA的密集染色表明，在周围的纤维囊和组织侵入支架区域中形成了成熟的血管。在糖尿病和非糖尿病小鼠之间，在毛细血管密度和被血管占据的面积上没有统计学上的显着差异。总而言之，本研究显示糖尿病对SC植入的明胶冷冻琼脂糖冷冻凝胶支架中新血管形成没有不利影响。

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