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首页> 外文期刊>Acta biomaterialia >Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.
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Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.

机译：在基于白蛋白的基质上图案化细胞共培养物的制备：微流体装置的应用。

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摘要

A surface coated with cross-linked albumin film resists the adhesion of cells, and subsequent exposure to UV irradiation or electrostatic adsorption of a cationic polymer switches the surface from non-adherent to adherent. Taking advantage of this unique property of cross-linked albumin, the authors fabricated patterned cell co-cultures with desired patterns and cell types. In this scheme, the cell-adherent region was initially created in the cell-non-adhesive albumin substrate, on which a first cell type was attached. Subsequently, the remaining region was also changed to adherent for the attachment of secondary cells in the same manner, thereby allowing distinctly localized co-cultures. As a proof of concept demonstration of the feasibility of this approach, a patterned co-culture of Neuro-2a cells with L929 cells was successfully prepared on the substrate. Furthermore, combining this technique with a microfluidic technique, a micropatterned co-culture of PA6 cells with 3T3 fibroblasts was created inside microfluidic devices. This approach could potentially be a useful tool for fundamental investigations of cell-cell interactions and for tissue engineering applications.
机译：涂有交联白蛋白膜的表面可抵抗细胞的粘附，随后暴露于紫外线辐射或阳离子聚合物的静电吸附会使表面从非粘附状态变为粘附状态。利用交联白蛋白的这种独特性质，作者制作了具有所需模式和细胞类型的带图案的细胞共培养物。在该方案中，最初在不粘附细胞的白蛋白基质中创建了细胞粘附区域，在该基质上附着了第一种细胞。随后，剩余的区域也以相同的方式变为贴壁，以附着二次细胞，从而实现了明显的局部共培养。作为此方法可行性的概念证明，成功在基板上制备了Neuro-2a细胞与L929细胞的模式共培养。此外，将该技术与微流体技术相结合，在微流体设备内部创建了PA6细胞与3T3成纤维细胞的微模式共培养。这种方法可能是用于细胞间相互作用的基础研究和组织工程应用的有用工具。

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